This study was implemented to verify effectiveness related to job stress, depression or fatigue of hospital workers, by let them wearing necklaces with essential oils injected which were extracted from herb plants, as an alternative therapy. For this study, totally 60 people were selected who work in K hospital located in D city, in accordance with their agreement with participation in, and who were divided into 30 and 30 for a control group and an experimental group as appropriate subjects. The study was followed for 2 weeks from June 29 of 2015 by July 10 of 2015, by letting the experimental group wearing essential oils necklaces with a distance of 20 cm from noses during working time for 8 hours per day. After inhaling essential oils by wearing the necklaces, the job stress status didn’t have obvious difference between two groups statically, such as 104.1±11.3 points from 101.2±10.3 points for the control group and 104.7±9.8 points from 104.9±9.0 points for the experimental group. In case of the depression status, the control group had no difference such as 28.8±8.0 points from 29.4±9.0 points, and the experimental group had small decrease with 0.017 points such as 26.6±5.3 points from 28.5±5.6 points. In case of the fatigue status, the control group had no difference such as 75.7±19.7 points from 72.0±19.0 points, and the experimental group had small decrease with 0.049 points such as 64.3±20.0 points from 70.5±19.0 points.