This research was carried out to improve the utilization of agar by evaluating the effect of agarooligosaccharides on the intestinal microflora. Medium containing 0.2% agarooligosaccharides remarkably enhanced the growth of Bifidobacterium infantis; however, agarooligosaccharides did not influence the growth of Clostridium perJlingens. Agarooligosaccharides affected intestinal microflora to different extent by various pH and NaCl concentration. The growth of B. infantis enhancad over pH 4.5. Within 1 % NaCl concentration, addition of agarooligosaccharides enhanced the growth of B. infantis. In contract, NaCl did not affect the growth of Cl. perflingens at all concentrations tested. Therefore, agarooligosacchariedes improved the benevolent intestinal microflora and depressed to the level of bacteria causing putrefaction and food poisoning.