Pectobacterium carotovorum subsp. carotovorum was found to be highly virulent to various vegetables, including Chinese cabbage. The antibacterial isolate CAB12243-2 was tested in a field bioassay for suppressing soft rot disease. The nucleotide sequencing of the 16S rRNA gene identified, the CAB12243-2 strain used in this study as Bacillus toyonensis. B. toyonensis CAB12243-2 inhibited the pectate lyase process by soft rot pathogens, and used trehalose and glucose as carbon sources. In field tests, the antibacterial isolate B. toyonensis CAB12243-2 suppressed soft rot disease with 73.0% control efficacy on the spring cultivar “Norangbom” and with 68.9% efficacy on the fall cultivar “Bulam 3”. These results suggest that B. toyonensis CAB12243-2 can be used as a biological control agent for the control of soft rot diseases on vegetables.

Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 무름병 억제 미생물 분리 및 선발
  2. 선발 미생물의 동정 및 특성검정
  3. 생물적 방제 효과 시험
 Ⅲ. 결과 및 고찰
  1. 무름병 억제 미생물 분리 및 선발
  2. 선발 미생물의 동정 및 특성검정
  3. 생물적 방제 효과 시험
 Ⅳ. 적 요
 References

• 김병련(충청남도농업기술원 친환경농업과) | Kim, Byung-Ryun Corresponding author
• 박명수(서울대학교 생명과학부) | Park, Myung-Soo
• 한광섭(충청남도농업기술원 친환경농업과) | Han, Kwang-Seop
• 함수상(충청남도농업기술원 친환경농업과) | Hahm, Soo-Sang
• 박인희(충청남도농업기술원 친환경농업과) | Park, In-Hee
• 송재경(국립농업과학원 농업미생물과) | Song, Jae-Kyeong