논문 상세보기
pp.447-456
본 연구는 딸기 품종의 유전적 변이를 DNA 수준에서 비교함으로써 육종의 기초 자료로 활용하기 위하여 딸기 품종 및 계통 50점을 대상으로 AFLP와 SSR 분석을 수행하였다. 총 27종의 AFLP 프라이머와 13종의 SSR 프라이머를 이용여 각각 407개와 42개의 다형성 밴드를 얻었다. 획득된 449개의 다형성 밴드를 이용하여 UPGMA 방식으로 유사도 및 집괴분석을 수행한 결과 유전적 유사도 0.654를 기준으로 6의 그룹으로 분류되었다. 국내 육
Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were utilized for evaluation of genetic diversity of 50 strawberry cultivars. Twenty-seven selective AFLP primer pairs generated a total of 407 polymorphic amplification products and 13 SSR primers produced 42 alleles. From UPGMA (unweighted pair-group method arithmetic average) cluster analysis by using 449 polymorphic bands, the strawberry cultivars were divided into six clusters by similarity index of 0.654. Most of Korean and Japanese cultivars were included in the sixth (VI) cluster. AC1466 and Harunoka were not clustered into any group, and the principal component analysis confirmed the pattern of the cluster analysis for those two varieties. Similarity values among the tested strawberry cultivars ranged from 0.531 to 0.951, and the average similarity value L was 0.658. This indicated that the 50 strawberry cultivars were closely related even though they developed from independent breeding programs, respectively. It was confirmed that there were a few genetic variations among the strawberry cultivars used for this study.
