This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined 18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex was more specific rather than macroscopic observation.

서 론
 재료 및 방법
  고래 회충 육안검사법
  Anisakis 유충의 DNA 추출
  중합효소연쇄반응(Polymerase chain reaction, PCR)
  제한효소절편길이다형성(Restriction fragment length polymorphism, RFLP)
 결과 및 고찰
  Anisakis 유충의 육안검사
  고래 회충 유충의 PCR-RFLP
 요약 및 정리
 참고문헌

• 강주희(중앙대학교 생활과학대학 식품영양학과) | Ju-Hee Kang
• 이민화(중앙대학교 생활과학대학 식품영양학과) | Min Hwa Lee
• 이강범(중앙대학교 생활과학대학 식품영양학과) | Kang-Bum Lee
• 최창순(중앙대학교 생활과학대학 식품영양학과) | Changsun Choi Correspondence