In this study, total mercury (THg) variations of Black-tailed Gull (Larus crassirostris) eggs laid on Baengnyeongdo, West Sea of Korea were compared between within- and among clutch in order to ensure statistical representativeness of an egg sampling method. In the ANOVA analysis, the THg variation (59%) among clutches was much higher than within-clutch (27%). This means that the mercury variation of sampled eggs was significantly influenced by the mercury variation among female individuals occupying different nests. In spite of relatively smaller variation of THg in the within-clutch, the THg concentration depending on an egg-laying sequence within a clutch significantly appeared higher in the first-laid eggs rather than the second-laid eggs. Therefore, it is necessary to take one egg from one nest in order to sample more nests in terms of better sampling representativeness. Also, one egg selection from one nest should be consistently applied in order to ensure comparability of mercury concentrations among monitoring sites and monitoring years.