현대인들의 건강에 대한 관심이 커짐에 따라 한약재 소비량이 지속적으로 증가하고 있다. 그러나 최근 기후변화와 국내 한약재의 높은 수입 의존도에 따라 유해물질에 대한 오염 위험이 높아지고 있다. 곰팡이독소는 한약재 중 안전성을 평가하는 주요한 항목 중 하나이다. 본 연구에서 498 건의 한약재에 대해 곰팡이독소 모니터링을 진행 하였다. 면역컬럼을 이용해 한약재 중 아플라톡신을 추출 하였으며, 추출한 아플라톡신은 HPLC-FLD 방법을 통해 분석하였다. 직선성, 회수율, LOD 및 LOQ를 통해 본 연구에 이용한 실험법 검증을 실시하였다. 39건에서 평균 7.670 μg/kg의 아플라톡신이 검출되었으며, 0.610~77.452 μg/kg의 검출범위를 보였다. 특히 현호색은 국내 아플라톡신 기준이 없으나, 시험에 사용된 39건 중 5건에서 14.9 ± 4.1 μg/kg의 높은 아플라톡신 농도를 보였다. 즉, 현호색에 대한 기준 설정이 시급히 요구되어지며, 선제적 관리를 위한 지속적인 모니터링이 필요할 것으로 사료되어진다.

The consumption of herbal medicines has been increasing with growing interest in health. However, due to recent climate change and the complex distribution process of herbal medicines with high import dependence the likelihood of contamination with mycotoxin has been increased. Mycotoxins are emerging as key indicators for ensuring safety of herbal medicines. A total of 498 herbal medicine samples were screened for mycotoxin contamination in this study. Aflatoxin in the herbal medicine samples was extracted by using immunoaffinity column, then the extracted aflatoxin was quantified via high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) method. The extraction method was verified by linearity, recovery, LOD and LOQ. Aflatoxins were detected in 39/498 samples in an average of 7.670 μg/kg (0.610-77.452 μg/kg range). Although safety standards for Corydalis Tuber is not currently available in korea, five of the 39 samples had high concentration of aflatoxins (average of 14.9 ± 4.1 μg/kg). In conclusion, it is urgent to establish safety criteria of aflatoxin in Corydalis Tuber. The results of the current study suggest that continuous monitoring is necessary for proactive management of herbal medicine safety.

Materials and Methods
  실험재료
  표준품 및 시약
  표준용액 조제
  정제용 칼럼 및 분석장비
  추출 및 정제
  HPLC 분석
 Results
  검량선
  회수율
  검출한계 및 검량한계
  유통 한약재 중 곰팡이독소 오염도
 Discussion
 국문요약
 Reference

• 이현경(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Hyun-Kyung Lee Correspondence to
• 김애경(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Ae-Kyeong Kim
• 김욱희(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Ouk-Hee Kim
• 김성단(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Sung-Dan Kim
• 이영주(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Young-Ju Lee
• 이새람(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Sea-Ram Lee
• 김일영(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Il-Young Kim
• 이정미(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Jung-Mi Lee
• 유인실(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | In-Sil Yu
• 정권(서울시보건환경연구원 한약재검사팀, Seoul Metropolitan Government Research Institute of Public Health and Environment, Herbal Medicine Inspection Team) | Kweon Jung