루이보스티에서 5종의 phenolic acid (gallic acid, chlorogenic acid, caffeic acid, p-coumaric acid, trans-ferulic acid) 와 9종의 flavonoid (procyanidin b1, aspalathin, rutin, vitexin, hyperoside, isoquercitrin, luteolin, quercetin, chrysoeriol)를 UPLC-MSMS를 이용하여 동시 분석하였다. 14종 페놀릭 류를 동시 분석하기 위하여 기기조건과 유효성을 검증하였고 확립된 분석방법을 이용하여 시중에 유통중인 루이보스티 30건을 채취하여 페놀릭류를 분석하였다. 루이보스티 1 g 혹은 1티백에 뜨거운 물 100 mL을 가하여 3분, 6분, 30분이 경과 후 그리고 차가운 물 (25-30oC)에 30분 우려낸 루이보스티의 페놀릭류 함량을 구하였다. 루이보스티에서 전체 실험대상 페놀릭류 중 rutin과 aspalathin이 가장 많이 추출되어 나왔으며 각각 물질의 함량은 제품별로 달랐다. 페놀릭류 성분의 추출효율은 14종 페놀릭류의 총 합 기준으로 뜨거운 물 30분 > 6분 > 3분 > 차가운 물 30분 순으로 높았다.

A simultaneous determination of 5 phenolic acids (gallic acid, chlorogenic acid, caffeic acid, pcoumaric acid, trans ferulic acid) and 9 flavonoids (procyanidin b1, aspalathin, rutin, vitexin, hyperoside, isoquercitrin, luteolin, quercetin, chrysoeriol) in rooibos tea has been carried out by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). A validated analysis method in this study was applied to rooibos aqueous infusions. Rooibos tea is an antioxidant-rich tea which has anti-cancer, anti-aging, anti-inflammatory, anti-diabetic effect. Extraction yield of phenolics depends on steeping time and temperature of water. Tea infusions were prepared by placing 1 g of tea leaves or 1 tea bag in 100 mL of boiled water, and then at 3, 6 and 30 minutes intervals the infused teas were taken to carry out the analysis of phenolic contents. Another tea infusion was conducted with cold water (25-30oC) for 30 minuntes. As a result, the total amount of phenolics was highest in rooibos tea steeped with hot water for 30 minutes, followed by 6 minutes, 3 minutes and cold water 30minutes and the result has statistical significance.

Materials and Methods
  시료
  표준 용액 및 시약
  분석기기 및 software
  시료전처리 방법
  분석물질의 정성을 위한 Ion ratio 구하는 방법
  분석방법의 유효성검증방법
  통계처리
 Results and Discussion
  TQD (MS/MS) detector 분석 조건설정
  분석방법의 유효성검증결과
 Conclusion
 국문요약
 References

• 박신희(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Sin-Hee Park Correspondence to
• 도영숙(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Yung-Suk Do
• 김윤성(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Youn-Sung Kim
• 김난영(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Nan-Young Kim
• 이진희(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Jin-Hee Lee
• 김종화(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Jong-Hwa Kim
• 윤미혜(경기도보건환경연구원 안양농수산물검사소, Anyang Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Korea) | Mi Hye Yoon