본 연구는 S. Typhimurium을 대상으로 paraformaldehyde 35%를 함유한 훈증소독제의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, S. Typhimurium의 현탁액 균수는 모두 3.9 × 108CFU/mL이었으며, 훈증소독제에 노출시킨 모든 담체의 균수는 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조담체로부터 회복된 S. Typhimurium 균수는 모두 3.3 × 106 CFU/mL이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 S. Typhimurium의 감소 균수는 5.22 log CFU/mL로 나타났다. 이상의 결과로부터, paraformaldehyde를 주성분으로 하는 훈증소독제는 S. Typhimurium에 대해 효과적인 살균력을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

This study was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 35% paraformaldehyde against Salmonella Typhimurium (S. Typhimurium). In this study, the efficacy test of a fumigant against S. Typhimurium was carried out according to French standard NF T 72-281. The S. Typhimurium working culture suspension number (N value), all bacteria numbers on the carriers exposed to the fumigant (n1, n2, and n3), the number of bacterial suspensions by the pour plate method (N1), the number of bacterial suspensions by the filter membrane method (N2), and the mean number of bacteria recovered on the control carriers (T value), were obtained from the preliminary test. In addition, the reduction number of S. Typhimurium exposed to the fumigant (d value) was calculated using the T value, the mean number of bacteria in the recovery solution (n'1) and the mean number of bacteria on carriers plated in agar (n'2). The N value was 5.5 × 108 colony forming units (CFU)/mL, and n1, n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, the T value was 3.5 × 106 CFU/carrier. In terms of the bactericidal effect of the fumigant, the d value was 5.25. According to the French standard for fumigants, the d value for an effective bactericidal fumigant should be greater than 5. The results indicated that the fumigant containing 35% paraformaldehyde had an efficient bactericidal activity against S. Typhimurium, and, therefore, can be used to disinfect food materials and kitchen appliances contaminated with foodborne bacteria.

Materials and Methods
  Fumigant
  Bacterial culture
  Dilution of bacterial suspension
  Counting the in-milk test suspensions
  Application of bacterial suspension to carriers
  Screening test for an inhibitory effect of fumigantresidues
  Evaluation of the fumigant in terms of bactericidalefficacy
  Experimental data and calculations
 Results and Discussion
  Screening test for an inhibitory effect of fumigantresidues
  Evaluation of the fumigant in terms of bactericidalefficacy
 Acknowledgement
 국문요약
 References

• Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Song-Ee Son(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Ji-Youn Jung(Department of Companion and Laboratory Animal Science, Kongju National University)
• Chang-Yeul Yoo(Department of Smart Information Convergence, Gyeongnam Provincial Namhae College)
• Eun-Kee Park(Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu-Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Correspondence to