논문 상세보기
pp.540-546
콩의 PR10 유전자(GmPR10)를 벼에 형질전환하여 GmPR10 전이 유전자의 발현 정도와 내염성 관련 형질의 반응 사이의 인과관계를 조사하여 염 스트레스에 대한 GmPR10 생리적 기능을 분석하고 내염성 유전자원을 개발하였다. 1. 전이 유전자는 형질전환 계통에 따라 게놈 내에 1 ~ 6개의 사본이 도입되었고, 선발된 8개의 형질전환 계통 모두에서 전이 유전자가 발현되었으며, 발현 정도는 계통에 따라 변이를 보였다. 2. T1세대 2계통의 형질전환
This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the T1 transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.
