Double-stranded RNA (dsRNA) has been applied to control insect pests due to its induction of RNA interference (RNAi) of a specific target gene expression. Bacterial expression and formulation of dsRNA led to minimizing dsRNA degradation. In our studies overexpression of dsRNA specific to chymotrypsin, essential protease for the survival of the beet armyworm, Sopodoptera exigua, (SeCHY2) in Escherichia coli HT115 (DE3), was toxic to larvae after oral administration. In the other view, silencing of the transformer2 (tra-2) gene that seems to performance as the genetic modification generate female development in several dipteran species including the striped fruit fly, Baetrocera scutellata can used to development for male-only release technique to control of this pest.

• Mohammad Vatanparast(Department of plant Medicals, Andong National University)
• Yonggyun Kim(Department of plant Medicals, Andong National University)