Gelechioidea is the superfamily that contains diverse groups comprising over 16,000 species in worldwide. It was estimated that only 25% of the species diversity of the Gelechioidea has been described. In this study, DNA barcoding was assessed based on cytochrome c oxidase subunit I (COI) for the 154 morphologically identified Korean gelechioids. The results of three molecular species delimitation methods were consistent for 72% with their morphological identifications. Based on molecular operational taxonomic unit (MOTU) estimation, we discussed on discordant groups including barcode sharing pairs and cryptic species.