This study was conducted to evaluate the microbiological hazard on nursery school children's hands and to investigate the reduction effect of hand washing education. A total of 59 nursery school children's hands were tested. The average number of total aerobic bacteria was 3.72 ± 0.38 log CFU/hand. Five children's hands(2 male and 3 female) were positive(14.3%) for the coliform bacteria. These results showed that hand washing education are required repetitively. Among the pathogenic bacteria tested in this study, Staphylococcus aureus and Bacillus cereus were detected in 9(25.7%) and 16(45.7%) out of 35 their hands, respectively. Twelve out of sixteen B. cereus isolates(70.0%) produced enterotoxin. The results indicate that the hand hygiene of nursery school children needs to be improved. Comparing before and after hand washing in educated and non-educated group, the reduction effect of total aerobic bacteria on their hands was 0.42 and 0.60 log CFU/hand, respectively. The educated group showed 0.18 log CFU/hand higher reduction effect than non-educated group but microorganism did not eliminate perfectly. From the results, using a hand sanitizer after washing with soap and the continuous hand washing education are required to control the contaminated bacteria on nursery school children's hands.

ABSTRACT
 서 론
 재료 및 방법
  연구대상 및 시료채취
  일반세균수 및 대장균군수 측정
  식중독 미생물 분리 동정
  독소 생산능 확인실험
  항생제 감수성 실험
  손 씻기 교육
  손 씻기 교육의 미생물 저감화 효과 실험
  통계처리
 결과 및 고찰
  어린이집 유아 손의 미생물 오염도
  어린이집 유아 손의 식중독 미생물 오염도
  식중독 미생물의 독소생산 특성 및 항생제 내성
  손 씻기 교육에 따른 미생물 저감화 효과
 요 약
 감사의 글
 참고문헌

• 김중범(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Jung-Beom Kim Correspondence to
• 허은선(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Eun-Seon Hur
• 강석호(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Suk-Ho Kang
• 김대환(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Dae-Hwan Kim
• 도영숙(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Young-Sook Do
• 박포현(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Po-Hyun Park
• 박용배(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Yong-Bae Park
• 윤미혜(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Mi-Hye Yoon
• 이정복(경기도보건환경연구원 보건연구부, Health Research Department, Gyeonggi-do Institute of Health & Environment) | Jong-Bok Lee