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복어 중 테트로도톡신 분석법에 관한 연구 KCI 등재

The Study on the Analysis Method of Tetrodotoxin in Puffer Fish

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

The current standard for testing tetrodotoxin (TTX) in foodstuffs is the mouse bioassay (MBA) in Korea as in many other countries. However, this test suffers from potential ethical concerns over the use of live animals. In addition, the mouse bioassay does not test for a specific toxin thus a sample resulting in mouse incapacitation would need further confirmatory testing to determine the exact source toxin (e.g., TTX, STX, brevotoxin, etc.). Furthermore, though the time of death is proportional to toxicity in this assay, the dynamic range for this proportional relationship is small thus many samples must be diluted and new mice be injected to yield a result that falls within the quantitative dynamic range. Therefore, in recent years, there have been many efforts in this field to develop alternative assays. High performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) has been emerged as one of the most promising options. A LC-MS-MS method involves solid-phase extraction (SPE) and followed by analysis using an electrospray in the positive ionization mode and multiple reactions monitoring (MRM). To adopt LC-MS-MS method as alternative standard for testing TTX, we performed a validation study for the quantification of TTX in puffer fish. This LC-MS-MS method showed good sensitivity as limits of detection (LOD) of 0.03~0.08 μg/g and limits of quantification (LOQ) of 0.10~0.25 μg/g. The linearity (r2) of tetrodotoxin were 0.9986~0.9997, the recovery were 80.9~103.0% and the relative standard deviations (RSD) were 4.3~13.0%. The correlation coefficient between the mouse bioassay and LC/MS/MS method was higher than 0.95.

목차
ABSTRACT
 서 론
 재료 및 방법
  실험재료
  표준품 및 시약
  테트로도톡신의 기기분석
   추출
   정제
   LC/MS/MS 분석
  테트로도톡신의 마우스실험
 결과 및 고찰
  테트로도톡신의 기기분석
   검량선 작성
   LC/MS/MS 분석
   Validation
  기기분석법과 동물시험법 데이터 상관성 분석
 결 론
 요 약
 참고문헌
저자
  • 강영운(식품의약품안전평가원 식품위해평가부 오염물질과, Food Contaminants Division, Food Safety Evaluation Department) | YoungWoon Kang
  • 이윤숙(식품의약품안전평가원 식품위해평가부 오염물질과, Food Contaminants Division, Food Safety Evaluation Department) | YoonSuk Lee
  • 박성국(식품의약품안전평가원 식품위해평가부 오염물질과, Food Contaminants Division, Food Safety Evaluation Department) | SungKug Park
  • 서정혁(식품의약품안전평가원 식품위해평가부 오염물질과, Food Contaminants Division, Food Safety Evaluation Department) | JungHeok Seo
  • 김미혜(식품의약품안전평가원 식품위해평가부 오염물질과, Food Contaminants Division, Food Safety Evaluation Department) | Meehye Kim Correspondence to