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농산물 중 Staphylococcus aureus의 분리를 위한 선택배지 평가 KCI 등재

Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird- Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat (60oC for 90s), and cold (−20oC for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

목차
ABSTRACT
 서 론
 재료 및 방법
  균주와 배지
  선택배지의 민감도와 특이도 분석
  선택배지의 회복능 조사
  농산물에서 회수율 조사
  통계처리
 결과 및 고찰
  민감도와 특이도분석
  선택배지의 회복능 및 농산물에서 회수능 조사
 요 약
 감사의 글
 참고문헌
저자
  • 김세리(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Se-Ri Kim
  • 이서현(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Seo-Hyun Lee
  • 서민경(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Min-Kyoung Seo
  • 김원일(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Won-Il Kim
  • 박경훈(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Kyeong-Hun Park
  • 윤혜정(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Hye-Jeong Yun
  • 윤요한(숙명여자대학교 식품영양학과, Department of Food &Nutrition, Sookmyung Women's University) | Yohan Yoon
  • 유순영(서울지방식약청, Seoul Regional Korea Food & Drug Administration) | Soon-Young Yoo
  • 류경열(농촌진흥청 연구정책국 평가관리과, R&D Evaluation Division, Research Policy Bureau, RDA) | Kyoung-Yul Ryu
  • 윤종철(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Jong-Chul Yun
  • 김병석(농촌진흥청 국립농업과학원 농산물안전성부 유해생물과, Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) | Byung-Seok Kim Correspondence to