This study attempted to establish an ultra performance liquid chromatography (UPLC) analysis method for standard determination of silymarin as a health functional food material in Silybum marianum extraxt (milk thistle). UPLC was performed on a Waters Acquity BEH C18 (50×2.1 mm, 1.7 μm) column using a gradient elution of distilled water and methanol at a flow rate of 0.21 mL/min and detection wavelength of 288 nm. The UPLC method showed high linearity in the calibration curve at a coefficient of determination (r2) of >0.9999, and limit of detection and quantitation for 6 flavonolignans were 0.0167-0.2469 and 0.1648-1.2931 μg/mL, respectively. The recovery of each flavnolignan was in the range of 99.96-100.81%, and the relative standard deviation for precision of each flavonolignan was less than 1.0%. The UPLC method established in this study was more specific for the quantitative determination of silymarin than the HPLC method. Also, since the UPLC method is shorter in the equipment operation time and smaller in the amount of used solvent than the HPLC method, UPLC is expected to have higher energy efficiency and lower environmental impact compared with HPLC.

Abstract
 서 론
 재료 및 방법
  재료 및 시약
  표준용액의 조제
  기기 조건 및 조작
  정량 계산
  분석법 검증
  건강기능성식품 내에서의 실리마린 분석
 결과 및 고찰
  특이성(specificity)
  직선성(linearity)
  정확성(Accuracy)
  정밀성(precision)
  완건성(robustness)
  검체 함량
 요 약
 감사의 글
 References

• 유동선(부광약품, 서울과학기술대학교, Bukwang Pharm. Co., Ltd, Korea Food Research Institute) | Korea Food Research Institute
• 정경희(한국식품연구원, Korea Food Research Institute) | Kyung Hee Jung
• 최승준(서울과학기술대학교 식품공학과, Department of Food Science and Technology, Seoul National University of Science and Technology) | Seung Jun Choi Corresponding author