논문 상세보기
pp.207-213
This study aimed to evaluate the genotoxicity of Litsea japonica fruit-hexane extract (LJF-HE). In order to examine the genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, and a micronucleus induction (MN) test according to the OECD and the Korea Ministry of Food and Drug Safety (MFDS) toxicity test guidelines. In the bacterial reverse mutation assay, no significant increase in revertant colonies, nor bacterial toxicity, was observed in the LJF-HE treatment group, regardless of the absence or presence of metabolic activation by the S9 mixture. However, in the positive control group, revertant colony counts were shown to be more than twice that of the negative control group. The chromosome aberration test showed that the repetition rate of abnormal chromosome aberration was less than 5%, regardless of the treatment time, and with or without the S9 mixture. No significant change was observed when (p < 0.05) compared with the negative control group. The micronucleated polychromatic erythrocytes (MNPCE) repetition rate of the polychromatic erythrocytes (PCE) showed no significant changes when compared with the negative control group (p < 0.05). The PCE portion of total erythrocytes also showed no significant changes (p < 0.05). These results showed that LJF-HE had no significant genotoxic effects.
Materials and Methods
까마귀쪽나무열매추출물(LJF-HE) 제조
까마귀쪽나무열매추출물(LJF-HE)의지표성분분석(Hamabiwalactone B) 함량분석
세균을 이용한 복귀돌연변이시험
염색체 이상 시험
마우스 골수세포를 이용한 소핵시험
통계학적 분석
Results and Discussion
까마귀쪽나무열매추출물(LJF-HE)의 지표성분 함량
복귀돌연변이시험
염색체 이상 시험
마우스 골수세포를 이용한 소핵시험
국문요약
References
