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까마귀쪽나무열매추출물의 유전독성 평가 KCI 등재

Genotoxicity Study of Litsea japonica Fruit Flesh Extract

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

This study aimed to evaluate the genotoxicity of Litsea japonica fruit-hexane extract (LJF-HE). In order to examine the genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, and a micronucleus induction (MN) test according to the OECD and the Korea Ministry of Food and Drug Safety (MFDS) toxicity test guidelines. In the bacterial reverse mutation assay, no significant increase in revertant colonies, nor bacterial toxicity, was observed in the LJF-HE treatment group, regardless of the absence or presence of metabolic activation by the S9 mixture. However, in the positive control group, revertant colony counts were shown to be more than twice that of the negative control group. The chromosome aberration test showed that the repetition rate of abnormal chromosome aberration was less than 5%, regardless of the treatment time, and with or without the S9 mixture. No significant change was observed when (p < 0.05) compared with the negative control group. The micronucleated polychromatic erythrocytes (MNPCE) repetition rate of the polychromatic erythrocytes (PCE) showed no significant changes when compared with the negative control group (p < 0.05). The PCE portion of total erythrocytes also showed no significant changes (p < 0.05). These results showed that LJF-HE had no significant genotoxic effects.

목차
ABSTRACT
 Materials and Methods
  까마귀쪽나무열매추출물(LJF-HE) 제조
  까마귀쪽나무열매추출물(LJF-HE)의지표성분분석(Hamabiwalactone B) 함량분석
  세균을 이용한 복귀돌연변이시험
  염색체 이상 시험
  마우스 골수세포를 이용한 소핵시험
  통계학적 분석
 Results and Discussion
  까마귀쪽나무열매추출물(LJF-HE)의 지표성분 함량
  복귀돌연변이시험
  염색체 이상 시험
  마우스 골수세포를 이용한 소핵시험
 국문요약
 References
저자
  • 윤지현((주)휴럼중앙연구소) | Ji-Hyun Yun (Hurum Central research institute Co., Ltd)
  • 박인재((주)휴럼중앙연구소) | In-Jae Park (Hurum Central research institute Co., Ltd)
  • 박성환((주)휴럼중앙연구소) | Sung-Hwan Park (Hurum Central research institute Co., Ltd)
  • 최구희((주)휴럼중앙연구소) | Goo-Hee Choi (Hurum Central research institute Co., Ltd)
  • 김현정((주)휴럼중앙연구소) | Hyun-Jung Kim (Hurum Central research institute Co., Ltd)
  • 조주현((주)휴럼중앙연구소) | Ju-Hyun Cho (Hurum Central research institute Co., Ltd) Correspondence to