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Effects of cryopreservation on equine adipose tissue-derived mesenchymal stem cell plasticity KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/351524
pp.1-9
Mesenchymal stem cells (MSCs), which are present in all tissues, can differentiate into cells with various specific functions. Recently, cell-based therapies using MSCs have been increasing in the veterinary research and related fields. In this study, we investigated the cellular morphology, proliferating capacities, expression of cell surface markers such as CD13, CD34, CD44, CD45, CD90, and CD105, mesodermal differentiation potentials, and expression of senescence-related markers of p53, p21, and telomerase reverse transcriptase in equine adipose tissue-derived MSCs (eAD-MSCs) after cryopreservation. The eAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 1 year (< 1 year, G1) and more than 3 years (> 3 years, G2), respectively. After cryopreservation for 1 - 3 years, G2 eAD-MSCs showed similar cellular morphology, proliferating capacities, and expression of cell surface markers when compared with G1 eAD-MSCs. Moreover, cryopreservation did not affect the adipogenic, chondrogenic, or osteogenic differentiation potentials of G1 and G2 eAD-MSCs. Collectively, cryopreservation for (or over) 3 years maintained the stem cell phenotype and differentiation potentials of eAD-MSCs. These results will be an advantage that can be effectively used for future development of cell-based therapies.
서 론
재료 및 방법
지방조직 유래 성체줄기세포 분리 및 배양
지방조직 유래 성체줄기세포의 동결 보존 및 융해
유세포 분석을 통한 세포 표면 특이 단백질 발현 검사
중배엽 분화 유도 및 염색
Quantitative real-time RT-PCR (qRT-PCR)을 이용한유전자 발현 검사
세포 노화와 관련된 β-galactosidase 염색
통계학적 분석
결 과
동결 보존이 성체줄기세포의 형태 및 증식능에 미치는 영향
동결 보존이 성체줄기세포의 세포 표면 특이 단백질발현 및 중배엽 분화능에 미치는 영향
동결 보존이 성체줄기세포의 세포 노화에 미치는 영향
고 찰
REFERENCES
