Highly pathogenic avian influenza virus (HPAIV) damages vital organs and tissues, frequently leading to death in birds, and causes serious economic losses in the poultry industry. In addition, HPAIV can infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Clean-Zone®, which contains citric acid, malic acid and phosphoric acid, against avian influenza virus (AIV, H9N2) was investigated. Virucidal efficacy was determined by examining the viability of AIV after contact with the disinfectant in the allantoic membrane of chicken embryos. The disinfectant and AIV were reacted under hard water (HW) and organic matter suspension (OM) condition. AIV was inactivated with 200- and 50-fold dilutions of the disinfectant under HW and OM conditions, respectively. As the disinfectant, Clean-Zone®, has a virucidal efficacy against AIV, it can be used to prevent the spread of animal viral diseases.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Disinfectant
  AIV and culture
  Diluents and treatment condition
  Virus-disinfectant contact reaction
 RESULT AND DISCUSSION
 REFERENCES

• Won-Seok Chae(Schools of Life-Chemistry, Daejin University)
• Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Chang-Yeul Yoo(Department of Smart Information and Convergence, Gyeongnam Provincial Namhae College,)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu-Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author