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Immunomodulatory effects of stem cell application in equine musculoskeletal disorders KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/351529
pp.28-36
Musculoskeletal disorders including fracture, tendonitis, osteoarthritis, and laminitis are common diseases in racehorses that can cause large economic losses in the racehorse industry. Mesenchymal stem cells (MSCs) are being applied as new clinical tools for treatment of musculoskeletal disorders of racehorses. To investigate the immunomodulatory effects of stem cell therapy, we analyzed the anti- and pro-inflammatory factors in peripheral blood mononuclear cells of racehorses before and after stem cell application using quantitative real-time RT-PCR. The expression levels of pro-inflammatory factors (CCL5, IFN-γ, IL-2, and IL-18) were decreased while those of anti-inflammatory factors (TIMP-1, IL-10, TGF-β1, and VEGF) were increased significantly after application of equine adipose tissue-derived MSCs (eAD-MSCs) to racehorses with fracture. Moreover, the expression levels of pro-inflammatory factors (IL-2, IL-18, and TNF-α) were decreased while those of anti-inflammatory factors (TIMP-1, TIMP-2, IL-10, TGF-β1, and VEGF) increased significantly after stem cell application of eAD-MSCs in racehorses with tendonitis. After evaluating immunomodulatory effects of stem cell therapy on equine musculoskeletal disorders such as fracture and tendonitis, our results showed that expression levels of pro-inflammatory factors were decreased, while those of anti-inflammatory factors increased significantly after stem cell application of eAD-MSCs. These findings suggest that the healing effects of the stem cell therapy might be due to its modulation of inflammatory factors.
서 론
재료 및 방법
지방조직 유래 성체줄기세포 분리 및 배양
유세포 분석을 통한 세포 표면 특이 단백질 발현 검사
중배엽 분화 유도 및 염색
동종 유래 성체줄기세포 시술 및 시료 수집
Quantitative real-time RT-PCR (qRT-PCR)을 이용한유전자 발현 검사
통계학적 분석
결 과
지방조직 유래 성체줄기세포 분리 및 특성 분석
고 찰
REFERENCES
