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Codonopsis lanceolata 분자 마커를 이용한 Codonopsis 속 식물의 다양성 분석

Diversity Analysis of Codonopsis Genus Using Molecular Markers Based on Codonopsis lanceolata

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  • URLhttps://db.koreascholar.com/Article/Detail/352121
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : The Codonopsis genus belongs to the Campanulaceae, and it is recorded that there are four species of Codonopsis genus in Korea, such as Codonopsis lanceolata, Codonopsis pilosula, Codonopsis minima, and Codonopsis ussuriensis. C. lanceolata has been proved to be safety and efficacy, and has been widely used for medicinal and edible purposes for a long time in East Asian countries including Korea, China and Japan. However, little genetic research has been done.
Methods and Results : Ten species of Codonopsis plants were collected and DNA was extracted using CTAB (cetyl trimethylammonium bromide) method. The extracted DNA was diluted to 5 ng/㎕ for the PCR (polymerase chain reaction) process. C. lanceolata genome was used to develop molecular markers by searching insertion and deletion regions (InDel) in the chloroplast sequence. The developed markers were applied to 4 individuals per Codonopsis species. PCR amplification was carried out using a denaturation at 94℃ for 30 sec, annealing at 58℃ for 30 sec and extension at 72℃ for 30 sec, repeated for 35 total cycles. The PCR products were separated in a 4% agarose gell at 100 V for 40 min.
Conclusion : Using the molecular markers developed in this study, genetic diversity of Codonopsis genus was tested, and at the same time, a specific molecular marker was developed to differentiate C. lanceolata from the Codonopsis plants.

저자
  • 이재복(충북대학교 특용식물학과) | Jae Bok Lee
  • 길진수(충북대학교 특용식물학과) | Jin Su Gil
  • 김세림(충북대학교 특용식물학과) | Se Rim Kim
  • 구성철(농촌진흥청 국립원예특작과학원) | Sung Cheol Koo
  • 김호방(바이오메딕 생명과학연구소) | Ho Bang Kim
  • 엄유리(국립산림과학원 산림약용자원연구소) | Yurry Um
  • 이이(충북대학교 특용식물학과) | Yi Lee Corresponding author