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젖산발효에 의한 약용작물 2종 추출물에 대한 항산화능 변화 연구

The Study on Changes of Antioxidant Capacities of Two Medicinal Crops Extracts by Lactic Acid Fermentation

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  • URLhttps://db.koreascholar.com/Article/Detail/352148
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : The development of an antioxidant to prevent disease by ROS-induced oxidative stress is necessary. This study investigated the changes of antioxidant capacities of two medicinal crops extracts by lactic acid fermentation.
Methods and Results : The changes of free-radical scavenging activity of medicinal crops extracts by lactic acid fermentation were evaluated by using DPPH free-radical scavenging assay and ABTS free-radical scavenging assay. The DPPH free-radical scavenging activity of extracts or lactic acid fermented extracts were estimated as followed. samples were thoroughly mixed with 1 ㎖ ethanol solution of 0.1 mM DPPH. After stand for 30 min in the dark, the absorbance was measured at 570 ㎚ by using a UV Spectrophotometer. ABTS scavenging activity of extracts or lactic acid fermented extracts were estimated as followed. The working solution was prepared by mixing 1 ㎖ of ABTS solution with 88 ㎖ of 50% ethanol. A total of 25 ㎕ of samples were mixed with 225 ㎕ of ABTS working solution and allowed to stand for 10 min. The absorbance was read at 732 ㎚ in a UV spectrophotometer. The data were showed that lactic acid fermented extracts were higher antioxidant ability than the extracts.
Conclusion : This study was showed that the antioxidant capacities of two medicinal crops extracts were improved by lactic acid fermentation.

저자
  • 이상혁(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Sang Hyuk Lee
  • 공룡(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Ryong Kong
  • 이재원(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Jae Won Lee
  • 최두진(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Doo Jin Choi
  • 이대영(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Dae Young Lee
  • 김금숙(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Geum Soog Kim
  • 이영섭(농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) | Young Seob Lee Corresponding author