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Experimental validation of optimal growth conditions for barley production in fresh forage growing system
Response Surface Methodology (RSM) is a widely used statistical analysis for finding an optimal point of a response variable (dependent variable) in accordance with multiple explanatory variables (independent variable). We have previously used RSM to identify optimal temperature and humidity (represented by the amount of water spray) of the fresh forage system for maximizing the growth of barley after 6 days of seeding. Consequently, RSM demonstrated that the optimal growth condition of barley was 19.70 and 1.38 l/h. However, it was not proved by empirical result, we aimed to verify the RSM result through comparative experiments. Experiments were designed four trials with different conditions. The first trial represented optimal conditions obtained from RSM, i.e., 20 and 1.4(l/h), while the rest of trials represented; 1) 20 and 1.8(l/h), 2) 18 and 1.4(l/h), and 3) 23 and 1.4(l/h). After 6 days of seeding, the average barley weight of the first trial was 11,094 g which was the highest yield compared to other trials (10,101 g, 9,757 g, and 10,233 g). This result supports that the RSM successfully predicted the optimal growth condition to be applied for the fresh forage system.
