The purpose of this study was to utilize the stem of Opuntia ficus-indica and to optimize the extraction conditions and standardize extract process for water soluble dietary fiber and solid. Extraction process was optimized by applying various conditions such as pH, ethanol concentration, extracting temperature and time. Maximum extraction yield for water soluble dietary fiber and solid were obtained using 100% water, indicating that ethanol hampered extraction. Also, extraction was unstable with higher heat, longer extraction time, and low acidic conditions. Maximum extraction of water soluble dietary fiber (27.92%) and solid (5.6 g) were obtained at 50°C, pH7, and 7 hr extraction time. Extract had antioxidant capacity at 1105.98 mg vitamin C equivalents (VCE) on ABTS assay and 118.10 mg VCE on DPPH assay.

• Chang-Hwan Lee(Department of Food Science and Biotechnology, Kyung Hee University)
• Moo-Yeol Baik(Department of Food Science and Biotechnology, Kyung Hee University)
• Young-Tae Ham(Department of Food Science and Biotechnology, Chung Ang University)
• Hae-Kyung Kim(Department of Food Science and Biotechnology, Han Seo University)
• Byung-Yong Kim(Department of Food Science and Biotechnology, Kyung Hee University)