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Stilbenoids are important bioactive constituents found in plants. However, their massive extraction from natural resources may cause high cost burden. Thus, the study was conducted to utilize tissue culture techniques for efficient and effective production of stilbenoids in blueberries. Firstly, callus tissues capable of producing stilbenoids were induced from leaf explant of blueberry plantlet and cultured both in the light and dark condition. Three callus lines were obtained after repeated subculture. The callus lines were suspended in MS liquid medium supplemented with 2,4-D (0.2 mg/ml) and BA(0, 0.1, 0.2 mg/ml). The growth index(GI) was determined for cell suspension cultures and the best GI was observed in light-grown yellow callus. Meanwhile, the highest stilbenoids levels were detected when dark-grown red callus was cultured in a liquid medium containing 2,4-D (0.2 mg/ml) with the exception of resveratrol. In particular, the pterostilbene content is 8.91 μg/g dry weight, which was amplified about 124 times as compared with the control (blueberry leaf).
