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Profiles of SULT1E1 expression in tiEsr1KO mice during folliculogenesis
Sulfotransferase 1E1 (SULT1E1, EST) is responsible for the sulfation and inactivation of betaestradiol at physiological concentrations. SULT1E1 null mice have reduced fertility by the disfunction of placenta and ovulation. Based our previous data it was revealed that the ovulation ability of 6-month old tiEsr1KO female mice is similar with the SULT1E1 female mice. In this study the possible relations in ovulation between estrogen and SULT1E1 was examined using real-time PCR and RIA methods in tiEsr1KO model mice. During the induction of superovualtion SULT1E1 gene expression peaked just before ovulation (6 hr posthCG injection) in control mice. As expected the expression patterns were similar between control and 4-weelk old tiEsr1KO female mice. Serum levels of E2 were increased in both tiEsr1KO mice and wild type mice but its level was higher 3 times in tiEsrKO mice than the wild type. Its levels became same between them after hCG administration. On the other hand, 6-month old mice shows the the dramatical increasing the SULT 1E1 expression during folliculogenesis, their expression was increased more than 100 times after hCG 6hr compared with PMSG 12hr control. In contrast with the 4-week old tiEsr1KO female mice, the expression levels of SULT1E1 gene expression were higher levels more than 20 times at 12 hr post PMSG injection. On the other hand, the expression levels of the other SULT family genes, SULT1A1 and SULT2A1 were very low compared with the SULT1E1 and did not show fluctuation during that period. Based on these results, it is suggested that the functional roles of estrogen during folliculogenesis may be regulated by SULT1E1through the metabolism of 17-beta estradiol and that the expression of SULT1E1 gene may be under the control of the levels of estrogen.
