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Variations of three Pollicipes mitella populations identified by genetic analysis
The turtle leg (Pollicipes mitella), which belongs to the family Pollicipes, is widely distributed in the rock along the shore areas of the southern sea, Jeju island and Ulleungdo island of Korean Peninsula. Generally, the size, type, stripe pattern, and color of this species vary in accordance with water depth, turbidity, nutrition, growth period, water temperature, and other environmental aspects. Genomic DNAs were isolated from 21 individuals of three turtle leg (P. mitella) populations of Tongyeong, Yeosu and Manjaedo located in the southern sea of the Korean peninsula. After incubation, we added 300 l of 3 M NaCl, and gently pipetted for a few minutes. 600 l of chloroform was then added to the mixture and inverted (no phenol). The DNA pellets were then incubation-dried for more than 10 hours, maintained at -40℃ until analysis, then dissolved in the ultra-pure water. The concentration of the extracted genomic DNA was measured by its absorbance ratio at 260 nm, with a spectrophotometer (Beckman DU 600 series, UK). Seven primers were exposed to generate the unique loci to each population and number of shared loci by the three populations of turtle leg which could be clearly scored. The hierarchical clustering tree was analyzed by the similarity matrices to generate a dendrogram using pc-package program Systat version 10. As regards average bandsharing value (BS) results, the higher fragment sizes (>1,200 bp) are much more observed in the Yeosu population. The number of unique loci to each population and number of shared loci by the three populations generated by PCR using 7 decamer primers in the turtle leg (P. mitella) population of Tongyeong, Yeosu and Manjaedo. Genetic distances among different individuals of the Tongyeong population of the turtle leg (lane 01~07), Yeosu population of the turtle leg (lane 08~14) and Manjaedo population of the turtle leg (lane 15~21), respectively, were generated using the CLASSIFICATION option in Systat version 10 according to the bandsharing values and similarity matrix. Tongyeong population could be evidently discriminated with the other two Yeosu and Manjaedo populations among three populations. The longest genetic distance (0.305) was found to exist between individuals' no. 02 of the Tongyeong population and no. 13 of the Yeosu population. It seems to the author that this is a result of a high degree of inbreeding in narrow region for a long while. Three turtle leg (P. mitella) populations can be clearly distinguished, especially, by their morphological characters and PCR-based approach.
