본 연구는 경기지역 도축돈 및 도축우의 폐렴병변에서 Mycoplasma spp.의 발생 분포를 조사하고자 수행하였다. 부천 소재 도축장에 출하된 소와 돼지의 폐에 대하여 육안적 검사를 하고, 이 중 병변을 보인 소 192두와 돼지 257두의 폐에 대한 PCR 검사 결과, Mycoplasma spp.는 소에서 147두(76.5%), 돼지에서는 203두(80.9%) 에서 각각 검출되었다. 소, 돼지 각각의 Mycoplasma spp.에 대한 세부 primer를 이용한 검사 결과에서는 소에서 M. agalactiae가 16두(8.3%)에서 검출되었으나, M. dispar, M. bovis 및 M. bovirhinis는 검출되지 않았다. 돼지에서는 M. hyo-pneumoniae가 74두(28.8%), M. hyorhinis가 13두(5.1%) 검출되었다. M. hyosynoviae는 검출되지 않았다. 본 연구를 통해 경기지역 도축우 및 도축돈에서 Mycoplasma성 폐렴이 상재하고 있음을 확인하였다.

The present study was conducted to investigate the prevalence of Mycoplasma spp. in cows and pigs with pneumonic lung lesions in Gyeonggi province in 2017. One hundred ninety two and 257 lung tissues were collected from slaughtered cows and pigs with pneumonic lung lesions, respectively, and examined for the These lung tissue samples were reviewed, analyzed, and examined for the presence of Mycoplasma spp. by a genus specific PCR. Among the examined animals,147 cows (76.5%) and 203 pigs (80.9%) were found to be infected with Mycoplasmaspp. The infected tissues were further examined to identify the specific species of Mycoplasma using species specific PCRs. The only identified species in cows was M. agalactiae which was detected from 16 cows (8.3%), whereas M. dispar, M. bovis, and M. bovirhinis were not detected. In pigs, M. hyopneumoniae was detected from 74 pigs (28.8%) and M. hyorhinis from 13 pigs (5.1%). M. hyosynoviae was not detected. Taken together, the current study indicates Mycoplasma spp. are commonly associated with lung infection in cows and pigs in Korea. Further studies are needed to evaluate the impact of mycoplasma infection on the development of lung diseases in farm animals.

ABSTRACT
 Materials and Methods
  샘플채취 방법
  DNA 분리
  PCR 수행
 Results and Discussion
 국문요약
 References

• 제미성(서울대학교 수의과대학 미생물학실) | Mi Seong Je (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
• 이찬희(서울대학교 수의과대학 미생물학실) | Chan-Hee Lee (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
• 김용백(서울대학교 수의과대학 임상병리학실) | Yongbaek Kim (Laboratory of Clinical Pathology, College of Veterinary Medicine, Seoul National University)
• 박건택(인제대학교 바이오테크놀러지학부) | Kun Taek Park (Department of Biotechnology, Inje University)
• 정우경(서울대학교 수의과대학 미생물학실) | Woo Kyung Jung (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) Correspondence to
• 박용호(서울대학교 수의과대학 미생물학실) | Yong Ho Park (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) Correspondence to