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Lateral flow immunoassay for analyzing sulfadimethoxine residues in muscle tissue of olive flounder (Paralichthys olivaceus) KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/356197
pp.175-179
Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for detecting antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 5-h treatment of samples by placing them in water dosed with sulfadimethoxine (SDM; 200 g/ton water), the residue depletion of SDM was investigated in 25 cultured olive flounders (Paralichthys olivaceus). Muscles from fish were sampled before treatment and on the 1st, 2nd, 3rd, 4th and 5th days after treatment. The concentration of SDM in the muscle was then determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the residue in olive flounder muscle. To investigate the recovery rate, standard solutions were added to muscle samples to obtain final concentrations of 25 and 50 ng/mL in the muscle. The recovery rates of all spiked samples were >96.6% of the spiked value. SDM was detectable in the muscle of fish treated with the drug until the 1st day of the withdrawal period. The present study shows that the LFIA can be easily adopted to detect SDM residues in the tissue of farmed fish.
INTRODUCTION
MATERIALS AND METHODS
Reagents and immunoassay kit
Drug treatment and collection of samples
Analysis of SDM residues in muscle samples
Preparation of standard curve
Recovery test
RESULTS
Standard curve of SDM
Analysis of SDM residues in muscle samples
DISCUSSION
REFERENCES
