In this study, we evaluated anti-inflammatory effect of biji in LPS-stimulated RAW264.7 cells. Biji inhibited the generation of NO and PGE2 through the suppression of iNOS and COX-2 expression. In addition, biji attenuated the expression of TNF-α and IL-1β induced by LPS. Biji blocked LPS-mediated IκB-α degradation and subsequently inhibited p65 nucleus accumulation in RAW264.7 cells, which indicates that biji inhibits NF-κB signaling. In addition, biji suppressed p38 phosphorylation induced by LPS. Our results suggests that biji may exert anti-inflammatory activity through blocking the generation of the inflammatory mediators such as NO, PGE2, iNOS, COX-2, TNF-α and IL-1β via the inhibiting the activation of NF-κB and p38. From these findings, biji has potential to be a candidate for the development of chemoprevention or therapeutic agents for inflammatory diseases.

Abstract
 서 언
 재료 및 방법
  실험재료
  비지 추출물 제조
  RAW264.7 세포배양
  Nitric oxide (NO) 측정
  Prostaglandin E2 (PGE2) 측정
  SDS-PAGE 및 western blot 분석
  Reverse transcriptase-polymerase chain reaction (RT-PCR)
  통계분석
 결과 및 고찰
  비지추출물의 iNOS와 COX-2 발현 억제를 통한 NO 및PGE2 생성억제효과
  비지추출물의 TNF-α와 IL-1β 발현 억제 효과
  비지추출물의 RAW264.7 세포에서 NF-κB의 활성 억제효과
  비지추출물의 RAW264.7 세포에서 MAPK의 활성 억제효과
 적 요
 References

• 박수빈(안동대학교 생약자원학과) | Su Bin Park (Department of Medicinal Plant Resources, Andong National University)
• 송훈민(안동대학교 생약자원학과, 국립백두대간수목원) | Hun Min Song (Department of Medicinal Plant Resources, Andong National University, Baekdudaegan National Arboretum)
• 김하나(안동대학교 생약자원학과) | Ha Na Kim (Department of Medicinal Plant Resources, Andong National University)
• 박광훈(국립산림과학원 산림약용자원연구소) | Gwang Hun Park (Forest Medicinal Resources Research Center, National Institute of Forest Science)
• 손호준(국립산림과학원 산림약용자원연구소) | Ho-Jun Son (Forest Medicinal Resources Research Center, National Institute of Forest Science)
• 엄유리(국립산림과학원 산림약용자원연구소) | Yurry Um (Forest Medicinal Resources Research Center, National Institute of Forest Science)
• 박지애(안동대학교 생약자원학과, 국립백두대간수목원) | Ji Ae Park (Department of Medicinal Plant Resources, Andong National University, Baekdudaegan National Arboretum)
• 정진부(안동대학교 생약자원학과, 안동대학교 농업과학기술연구소) | Jin Boo Jeong (Department of Medicinal Plant Resources, Andong National University, Agricultural Science and Technology Research Institute, Andong National University) 교신저자