Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin present in grapes, exerts a variety of actions to reduce superoxides, prevents diabetes mellitus, and inhibits inflammation. Resveratrol acts as a chemo-preventive agent and induces apoptotic cell death in various cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is unclear. In this study, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. Resveratrol significantly accelerated mineralization as compared with the control culture in differentiation of MDPC-23 cells. Resveratrol significantly increased expression of ALP mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly accelerated expression of ColⅠmRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly increased expressions of DSPP and DMP-1 mRNAs as compared with the control in differentiation of MDPC-23 cells. Treatment of resveratrol did not significantly affect cell proliferation in MDPC-23 cells. Results suggest resveratrol facilitates odontoblast differentiation and mineralization in differentiation of MDPC-23 cells, and may have potential properties for development and clinical application of dentin regeneration materials.

서 론
 재료 및 방법
  1. 실험재료
  2. 세포주와 세포배양
  3. Alizarine red-S 염색을 통한 골기질 형성 분석
  4. 역전사 중합효소연쇄반응(RT-PCR) 분석
  5. MTT 분석에 의한 세포증식 측정
  6. 실험자료의 통계학적 검정
 결 과
  1. MDPC-23 상아질모세포 분화과정에서 세포외 기질형성에 미치는 resveratrol의 효과
  2. MDPC-23 상아질모세포 분화과정에서 분화 특이유전자들의 발현에 미치는resveratrol의 효과
  3. MDPC-23 상아질모세포 증식에 미치는 resveratrol의 효과
 고 찰
 References

• Sang Hun Shin(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Jae-Sung Kim(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Su-Gwan Kim(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Dae-San Go(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Sun-Kyoung Yu(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Chun Sung Kim(Oral Biology Research Institute, School of Dentistry, Chosun University)
• Joo-Cheol Park(Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University)
• Do Kyung Kim(Oral Biology Research Institute, School of Dentistry, Chosun University) Correspondence to