The present study was conducted to investigate effects of rabbit meat extract on energy metabolism and muscle differentiation in C2C12 myotubes. Water extract of rabbit meat (10, 50, 100, and 200 μg/ml) was used to treat differentiated C2C12 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis were used to determine mRNA or protein levels of energy metabolism-related genes. Total adenosine triphosphate (ATP) content was also measured. Treatment with rabbit meat extract significantly increased expression levels of muscle differentiation markers (myogenin and myosin heavy chain) and mitochondrial biogenesis regulators (PGC1α, NRF1, and TFAM) in C2C12 myotubes compared to non-treated control. Additionally, rabbit meat extract activated phosphorylation of AMPK and acetyl-coA carboxylase (ACC). Rabbit meat extract significantly increased ATP contents in myotubes. These results suggest that rabbit meat extract has the potential to improve energy metabolism in skeletal muscles.

Sestrin 2 (SESN2) is a member of the sestrin family of stress-induced proteins that negatively regulate agingassociated biological processes. This study aims to investigate the role of SESN2 in regulating the differentiation potential and senescence of mesenchymal stem cells (MSCs) derived from young and elderly donors. Bulk RNA sequencing revealed a common decline in the SESN2 mRNA levels in MSCs from elderly individuals, which was confirmed via reverse transcription-polymerase chain reaction and western blot analyses. SESN2 knockdown in MSCs from young donors resulted in phenotypic changes similar to those in MSCs from elderly donors, including an enhanced expression of senescence and adipogenic markers and diminished expression of osteogenic markers. To confirm the effect of decreased SESN2 expression on osteogenic and adipogenic differentiation, we induced Sesn2 knockdown in mouse bone marrow-derived MSCs. Sesn2 knockdown suppressed the mRNA expression of osteogenic marker genes, alkaline phosphatase activity, and matrix mineralization. Furthermore, Sesn2 knockdown enhanced mRNA expression of the adipogenic marker genes and intracellular lipid accumulation. These results suggest that a decline in SESN2 expression during aging contributes to the shift of MSC differentiation from osteogenic to adipogenic lineage.

Background: In healthy dentin conditions, odontoblasts have an important role such as protection from invasion of pathogens. In mammalian teeth, progenitors such as mesenchymal stem cells (MSCs) can migrate and differentiate into odontoblast-like cells, leading to the formation of reparative dentin. For differentiation using stem cells, it is crucial to provide conditions similar to the complex and intricate in vivo environment. The purpose of this study was to evaluate the potential of differentiation into odonto/ osteoblasts, and compare co-culture with/without epithelial cells. Methods: MSCs and epithelial cells were successfully isolated from dental tissues. We investigated the influences of epithelial cells on the differentiation process of dental pulp stem cells into odonto/osteoblasts using co-culture systems. The differentiation potential with/without epithelial cells was analyzed for the expression of specific markers and calcium contents. Results: Differentiated odonto/osteoblast derived from dental pulp tissue-derived mesenchymal stem cells with/without epithelial cells were evaluated by qRT-PCR, immunostaining, calcium content, and ALP staining. The expression of odonto/ osteoblast-specific markers, calcium content, and ALP staining intensity were significantly increased in differentiated cells. Moreover, the odonto/osteogenic differentiation capacity with epithelial cells co-culture was significantly higher than without epithelial cells co-culture. Conclusions: These results suggest that odonto/osteogenic differentiation co-cultured with epithelial cells has a more efficient application.

서양 실내악의 쟝르인 예술가곡(藝術歌曲)은 성악작품으로 18세기말에서 19세기 초 유럽에서 탄생되었다. 음악과 시, 반주 가 고도로 결합되어 구성된 것이다. 독일과 오스트리아 (German and Austria)파 작곡가들인 슈베르트, 슈만, 브람스 (Johann Jakob Brahms, 1806–1872) 등이 이들의 발전에 크게 기여하였다. 사회의 발전과 더불어 부동한 나라와 지역에서 예 술가곡이 전파발전함에 따라 각자의 문화적 차이와 음악적 특 색을 나타내고 있다. 본문에서는 음악 창작의 차원에서 한국의 <마중>과 중국의 <친문타타하(親吻沱沱河)>에서 나타난 곡조 구조와 리듬 등에 대하여 분석과 연구하였다. 여기 한·중 예술 가곡은 각기 한 지역의 문화 특색과 예술을 내포하고 있어 양 국의 예술가곡 창작과 발전을 위한 일정한 계시와 공헌을 하였 음을 알 수 있었다.

작물을 재배하는 데 필요한 여러 가지 환경 조건 중 광은 개 화와 밀접한 연관이 있다. 본 연구는 식용화, 매리골드 화아분 화에 영향을 주는 최적의 광주기를 구명하여 완전제어형 식물 공장에서 효율적으로 재배하기 위해 진행되었다. 실험에 사 용된 광주기는 4, 8, 12, 16시간, 총 4가지로 설정하였다. 매리 골드 ‘듀란고 레드’ 종자를 우레탄 스펀지에 파종한 직후부터 광주기를 처리하였다. 화아분화는 꽃봉오리가 약 2mm 이상 일 때 화아분화가 되었다고 정의하였고, 2－3일 간격으로 조 사하였다. 생육 조사는 지상부의 생체중, 건물중, 초장, 엽면 적을 조사하였다. 최적의 광주기는 식물체의 50%가 화아분 화 된 날을 기준으로 정의하였다. 4시간 처리구에서는 식물체 가 제대로 자라지 못하며 화아도 형성되지 않았다. 8시간 이상 의 처리구에서부터 식물체가 정상적으로 생장하고 화아분화 가 이루어졌지만, 8시간 처리구는 12시간 이상의 처리구에 비 해 화아분화가 더디게 일어났다. 반면에 12시간 처리구와 16 시간 처리구는 서로 유의하지 않은 결과를 보였다. 모든 생육 조사 항목에서 16시간 광주기 처리구가 가장 높은 값을 나타 냈으나 지상부의 건물중과 엽면적을 제외한 나머지 항목에서 12시간 처리구와 유의하지 않았다. 실험 결과에 따르면, 8시간 광주기에서도 화아분화가 일어났지만, 화아형성까지의 시 간이 12시간 이상의 광주기일 때보다 더 많이 소요되었으며, 식물체의 생육 또한 12시간 이상의 광주기를 조사받은 식물 체보다 낮게 나타났다. 본 실험에서 에너지 소비량을 고려한 최적의 매리골드 ‘듀란고 레드’의 광주기는 12시간으로 판단 된다.

This study aims to distinguish between various Ethiopian durum wheat varieties based on their genetic identity using chemical and morphological characterization of seeds. Combinatorial employment of five chemical tests on seeds showed marked qualitative variation among the test varieties, with high discriminatory potential noted for the standard phenol test, followed by the modified phenol and iodide tests. The modified phenol test was instrumental in further discriminating between the varieties that were not identified using the standard phenol test. Unlike the iodide and phenol tests, the NaOH and KOH tests did not show significant variation among the varieties. These results underscore the efficacy of phenol and iodide tests in differentiating between durum wheat varieties. Although the morphological traits were advantageous in seed characterization, they lacked discriminatory power compared with that of the chemical tests. This study concludes that a single test is inadequate for varietal discrimination; rather, a combination of chemical tests can augment the discriminatory potential.

In this review, the regulatory mechanisms of autophagy were described, and its interaction with apoptosis was identified. The role of autophagy in embryogenesis, tooth development, and cell differentiation were also investigated. Autophagy is regulated by various autophagy-related genes and those related to stress response. Highly active autophagy occurrences have been reported during cell differentiation before implantation after fertilization. Autophagy is involved in energy generation and supplies nutrients during early birth, essential to compensate for their deficient supply from the placenta. The contribution of autophagy during tooth development, such as the shape of the crown and root formation, ivory, and homeostasis in cells, was also observed. Genes control autophagy, and studying the role of autophagy in cell differentiation and development was useful for understanding human aging, illness, and health. In the future, the role of specific mechanisms in the development and differentiation of autophagy may increase the understanding of the pathological mechanisms of disease and development processes and is expected to reduce the treatment of various diseases by modulating the autophagic phenomenon.

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

Background: Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer the immense therapeutic potential in stem cell-based therapy of degenerative disorders. However, clinical trials of human ESCs cause heavy ethical concerns. With the derivation of iPSCs established by reprogramming from adult somatic cells through the transgenic expression of transcription factors, this problems would be able to overcome. In the present study, we tried to differentiate porcine iPSCs (piPSCs) into endothelial cells (ECs) for stem cell-based therapy of vascular diseases. Methods: piPSCs (OSKMNL) were induced to differentiation into ECs in four differentiation media (APEL-2, APEL-2 + 50 ng/mL of VEGF, EBM-2, EBM-2 + 50 ng/ mL of VEGF) on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Differentiation efficiency of these cells were exanimated using qRT-PCR, Immunocytochemistry, Western blotting and FACS. Results: As results, expressions of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) were higher observed in all porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media than piPSCs as the control, whereas endothelial-associated marker (CD-31) in the differentiated cells was not expressed. Conclusions: It can be seen that piPSCs (OSKMNL) were not suitable to differentiate into ECs in the four differentiation media unlike porcine epiblast stem cells (pEpiSCs). Therefore, it would be required to establish a suitable PSCs for differentiating into ECs for the treatment of cardiovascular diseases.

인구와 경제가 집중되고, 문화와 여가 등 다양한 분야에서 핵심적인 역할을 수행하는 서울시는 이면에 공간 불평등으로 인한 문제가 대두되고 있다. 이 연구는 서울시의 공간 불평등을 규명하기 위한 첫 단계로 상류층과 빈곤층의 주거지 분화를 탐색하는 데 목적을 두었다. 주거지 분화를 규명하기 위해 아파트와 연립다세대 실거래가･공동주택가격･개인 소득 자료가 활용되었으며, 분석 방법으로 커널밀도추정을 이용한 핫스팟 분석이 수행되었다. 분석 결과 상류층 주거지와 빈곤층 주거지는 계층 간 분리와 계층 내 집중이라는 공간적 분포 패턴이 확인되었다. 상류층 주거지는 강남구를 위시한 소위 강남3구에, 빈곤층 주거지는 도시 외곽에 집중되어 있었다. 주거지 분화와 이로 인해 발생하는 여러 분야의 불평등에 관한 연구가 지속적으로 이루어질 필요가 있으며, 이 연구가 주거지별 격차와 공간 불평등을 다루는 연구의 초석이 되길 기대해 본다.

Background: Germ cells undergo towards male or female pathways to produce spermatozoa or oocyte respectively which is essential for sexual reproduction. Mesenchymal stem cells (MSCs) have the potential of trans-differentiation to the multiple cell lineages. Methods: Herein, rat MSCs were isolated from bone marrow and characterized by their morphological features, expression of MSC surface markers, and in vitro differentiation capability. Results: Thereafter, we induced these cells only by retinoic acid supplementation in MSC medium and, could able to show that bone marrow derived MSCs are capable to trans-differentiate into male germ cell-like cells in vitro. We characterized these cells by morphological changes, the expressions of germ cell specific markers by immunophenotyping and molecular biology tools. Further, we quantified these differentiated cells. Conclusions: This study suggests that only Retinoic acid in culture medium could induce bone marrow MSCs to differentiate germ cell-like cells in vitro . This basic method of germ cell generation might be helpful in the prospective applications of this technology.

Bioactive flavonoids have been shown to improve the biological activity of stem cells derived from different sources in tissue regeneration. The goal of this study was to see how naringin, a natural flavonoid discovered in citrus fruits, affected the biological properties of human dental pulp stem cells (HDPSCs). In this study, we found that naringin increases the migratory ability of HDPSCs. Naringin increased matrix metalloproteinase-2 (MMP-2) and C-X-C chemokine receptor type 4 (CXCR4) mRNA and protein expression in HDPSCs. ARP100, a selective MMP-2 inhibitor, and AMD3100, a CXCR4 antagonist, both inhibited the naringin-induced migration of HDPSCs. Furthermore, naringin increased osteogenic differentiation of HDPSCs and the expression of the osteogenic-related marker, alkaline phosphatase in HDPSCs. Taken together, our findings suggest that naringin may be beneficial on dental tissue or bone regeneration by increasing the biological activities of HDPSCs.

The differentiation of Chinese characters is accompanied by the development of Chinese writing and is one of the manifestations of the adaptation of Chinese characters to record Chinese language. There are not only macroscopic and microscopic reasons, but also textual factors and linguistic factors for the differentiation of Chinese characters. To record Chinese accurately and clearly is the radical cause of character differentiation. The emergence of specific differentiated characters has its own influencing factors. It is particularly important to investigate the lexical items and their properties recorded by the mother characters to uncover the specific causes of the differentiation. In terms of lexical items, the degree of meaning clarity, semantic alienation and that of prominence and emphasis are the important factors that affect whether the lexical items represented by the mother character can obtain graphic marks independently, which is helpful to explore the internal motivation of Chinese character differentiation.