간행물
한국동물생명공학회지 (구 한국수정란이식학회지) KCI 등재 Journal of Animal Reproduciton and Biotechnology
- 발행기관 한국동물생명공학회(구 한국수정란이식학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2671-4639 (Print)2671-4663 (Online)
- 수록기간 1983 ~ 2025
- 주제분류 자연과학 > 생물공학 자연과학 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Vol.40 No.4 (2025년 12월) 12건
1.
2025.12
구독 인증기관 무료, 개인회원 유료
Artificial insemination (AI) is a widely used assisted reproductive technology in livestock, but its commercialization in dogs remains limited. With the growing demand in the pet industry, it is necessary to evaluate the efficiency and commercial potential of canine AI in South Korea. A literature review was conducted using PubMed and Google Scholar, focusing on studies published between 1990 and 2025. Fourteen relevant papers were analyzed by semen type (fresh, chilled, frozen) and insemination technique (intravaginal, intrauterine, surgical). Cost data were collected from seven overseas AI service providers between February and May 2025, covering insemination procedures and breeding timing tests. The average pregnancy and delivery rates were evaluated using two denominators: inseminations and bitches. In fresh semen, the pregnancy and delivery rates were 94.4% for both (based on inseminations), 81.1% and 83.7% (based on bitches). In chilled semen, the pregnancy and delivery rates were 95.0% for both (inseminations), 62.4% and 55.8% (bitches). In frozen semen, rates were 73.1% for delivery (inseminations) and 65.5% and 58.3% for pregnancy and delivery (bitches). Among AI techniques, intravaginal insemination rates were 58.9% for delivery (inseminations) and the pregnancy and delivery rates were 62.4% and 57.1% (bitches). In intrauterine insemination, the pregnancy rates and delivery rates were 81.8% and 86.0% (inseminations), 87.5% for both (bitches). In surgical insemination, the pregnancy rates and delivery rates were 45.0% and 73.1% (inseminations), 100.0% and 73.5% (bitches). Service costs varied among providers: intravaginal ($137), intrauterine ($345-371), and surgical ($632-777), progesterone testing ($82) and vaginal cytology ($78). Service costs varied widely among providers: intravaginal insemination averaged $137, intrauterine $345-371, and surgical $632- 777. Additional expenses included progesterone testing ($82) and vaginal cytology ($78). This study analyzes the reproductive efficiency of various canine AI methods based on experimental findings reported in the literature and examines the service models of commercially operating providers, thereby providing foundational data for the potential commercialization of canine AI technology in South Korea.
4,200원
2.
2025.12
구독 인증기관 무료, 개인회원 유료
Islam M. Saadeldin, Ahmad Yar Qamar, Yunju Ha, Seeun Kim, Seungjun Lee, Sang-Gon Shin, Kiyoung Yoon, Hyejin Kim, Min Jung Kim, Jongki Cho
Developing reliable models that capture the complexity of maternal– embryonic interactions and implantation is essential for deepening our understanding of early embryonic development as well as the underlying mechanisms of reproductive disorders. Conventional two-dimensional (2D) culture systems, however, fall short in accurately replicating the dynamic and multilayered in vivo microenvironment. In contrast, three-dimensional (3D) organoid technologies have recently emerged as a transformative approach, offering structurally and functionally relevant platforms that better reflect physiological conditions. This review highlights the latest strategies, innovations, and methodological advances in employing 3D organoids to model maternal–embryonic communication and implantation processes. It also outlines their growing potential in research applications and personalized medicine, particularly within the context of assisted reproductive technologies. Furthermore, the review discusses future directions for organoid-based studies from the endometrium, oviduct, ovary, testis, and epididymis have been established, their integration with gametes and embryos marks a new frontier. Cross-species work, especially your pioneering studies on endometrial organoids and lacunoids/cystoids, opens translational opportunities for understanding maternal–embryonic crosstalk, implantation biology, and fertility disorders. The review will discuss current advances, technical challenges, and future directions toward interconnected organoid systems (organoid-on-chip).
4,600원
3.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Artificial insemination (AI) relies on liquid storage of boar semen for handling and timing to improve reproductive efficiency. However, storage promotes reactive oxygen species (ROS) accumulation in sperm, which damages the membranes, mitochondria, and DNA, leading to a loss of motility and integrity. The addition of antioxidants is a practical method for preventing ROS generation and functional loss. Accordingly, the effects of 1 nM Mitoquinone (MitoQ) on the viability, motility, and sperm protein function of boar sperm were evaluated. Methods: Semen extended with sperm was stored at 4℃ for up to 7 days in the presence of 1 nM MitoQ. Viability was assessed using SYBR-14/PI, and motility and kinematics (ALH, BCF, LIN, STR, VAP, VCL, VSL, and WOB) were measured using the computer-assisted sperm analysis (CASA) system. Western blotting was used to quantify phospho-AMPK (Thr172), total AMPK, and β-actin with α-tubulin as a loading reference. Results: Viability was higher in MitoQ from days 3 to 7 (p < 0.001). Total motility was also higher on day 1 (p < 0.01) and was better preserved on days 3,5 (p < 0.05) and 7 (p < 0.001). Kinematics showed reduced ALH and increased BCF with MitoQ, with higher LIN (day 3, p < 0.05) and STR (days 5 and 7, p < 0.05). VAP and VCL were transiently higher on day 1 and then lower than those of the control thereafter, whereas VSL and WOB were unchanged. The phospho-AMPK/total-AMPK ratio was higher with MitoQ on all measurement days (p < 0.001). β-actin did not differ on day 1, showed a numerical increase on day 3, and was higher on day 5 (p < 0.05). Conclusions: Viability and motility were preserved with 1 nM MitoQ, and sperm swimming was straighter and more efficient. The higher phospho-AMPK/total-AMPK ratio, together with preserved β-actin, points to AMPK-based energy control and a stable cytoskeleton. This supports follow-up studies on the dose, extender conditions, and fertility outcomes of boar artificial insemination.
4,000원
4.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Cows have an increased need for energy after calving due to milk production for nursing calves and the recovery of reproductive performance. Blood metabolites are associated with the reproductive ability of primiparous Hanwoo cows, but the relationship between changes in blood metabolite concentrations and reproductive ability remains unclear. The present study investigated the relationship between changes in blood metabolites from 2 to 5 weeks after calving and the recovery of reproductive ability in primiparous Hanwoo cows. Methods: Blood samples were obtained from 26 primiparous Hanwoo cows of average body condition score 3.0 (range, 1-5) weekly from 2 to 7 weeks postpartum and at the time of estrus, and the concentrations of blood metabolites were measured. Changes in blood metabolite concentrations were found to be associated with the recovery of reproductive function after calving. Results: The concentrations of non-esterified fatty acids (NEFA) and blood urea nitrogen (BUN) 3 to 4 weeks after calving were significantly associated with the first days of ovulation. Ovulation resumed when the average NEFA concentration reached 199.0 μEq/L, and the first normal estrus cycle was established when the average total cholesterol (T-Chol) concentration reached 169.1 mg/dL. Conclusions: Metabolic status 3 to 4 weeks postpartum influenced the resumption of estrus and ovulation in primiparous Hanwoo cows. Furthermore, increased T-Chol concentration was associated with the initiation of a normal estrus cycle.
4,200원
5.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Native Mongolian cattle have undergone long-term adaptation to the extreme continental climate of Central Asia, resulting in unique genetic characteristics shaped by natural selection and traditional pastoralist breeding practices. However, comprehensive genetic characterization of these breeds remains limited. Methods: We assessed the genetic diversity and population structure of three Mongolian cattle breeds, Mongol Cattle (MC), Dornod Tal (DT), and Dadal Ukhaa (DU), raised in three distinct regions of Mongolia, using 11 microsatellite markers. Results: Across all breeds, the mean number of alleles was 7.394, ranging from 6.545 in MC to 8.455 in DT. DT exhibited the highest expected heterozygosity (0.763) and polymorphic information content (0.719). Multivariate analyses, including principal coordinate analysis, factorial correspondence analysis, and STRUCTURE clustering, revealed a close genetic relationship between MC and DT, whereas DU was genetically distinct. Conclusions: This study comprehensively assessed the genetic diversity and structure of three Mongolian cattle breeds using microsatellite markers. The results revealed substantial within-breed variation and distinct genetic structuring, particularly in DU and DT, underscoring the importance of conserving their unique genetic heritage. These microsatellite findings provide essential baseline information, while future SNPbased genomic analyses with broader sampling will be crucial to support sustainable breeding, conservation, and effective management of native Mongolian cattle.
4,000원
6.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: In mammals, DRP1 is a key regulator of mitochondrial fission during mitochondrial dynamics, whereas ATF5 promotes the mitochondrial unfolded protein response (UPRmt). Both pathways are essential for maintaining cellular homeostasis and protecting oocytes and embryos from external stressors. However, the relationship between ATF5 expression and DRP1 under heat stress conditions during porcine oocyte maturation remains unclear. Methods: In this study, we investigated the mitochondrial dynamics and ATF5 expression in porcine oocytes exposed to heat stress during in vitro maturation (IVM). Protein and gene expression levels were assessed using immunofluorescence staining, Western blotting, and quantitative PCR. Results: During IVM, both DRP1 and ATF5 expression were increased (p < 0.01) significantly. In contrast, heat stress markedly impaired (p < 0.05) meiotic progression and cumulus cell expansion. Mitochondrial dynamics were disrupted (p < 0.05), as fission and fusion markers displayed reciprocal changes relative to those in controls. Concomitantly, the expression of ATF proteins was significantly reduced (p < 0.01) under heat stress. Heat-stressed oocytes also exhibited decreased (p < 0.05) expression of genes involved in antioxidant defense and NAD metabolism, whereas autophagy- and apoptosis-related transcripts were significantly upregulated (p < 0.05). At the blastocyst stage, embryos derived from heat-stressed oocytes exhibited nuclear localization of the UPR-associated transcription factors ATF4, CHOP, and ATF5. Conclusions: Collectively, our findings suggest that heat stress disrupts mitochondrial dynamics and ATF5 expression during porcine oocyte maturation while the UPRmt pathway remains active during early embryonic development to mitigate heat-induced cellular damage.
4,500원
7.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Pyometra, involves pus accumulation that can lead to death if detected late, making early diagnosis and management crucial, and it is more common in older dogs. The Axiom™ Canine HD Array was used in the study to determine single nucleotide polymorphisms (SNPs) associated with pyometra, with the aim of providing foundational data for the future development of SNP markers. Methods: Samples were collected from 95 dogs of 26 breeds in South Korea, and SNP genotypes were analyzed for the final two SNPs (AX-168186923, AX-168208364). To verify the sequences and genotypes of the selected SNPs, we conducted a sequencing analysis of 96 dogs from 11 breeds previously reported to have been affected by the disease. To confirm the linkage disequilibrium (LD) between the selected SNPs and the influence of non-synonymous SNP (nsSNP), candidate gene function and, protein structure change prediction analyses were performed. Results: Sequence and genotype analyses of the selected SNPs revealed differences in genotype frequencies among the varieties. A strong LD block was formed between the SNPs, confirming that both SNPs were genetically associated. Candidate gene ABCC4 analysis revealed that ABCC4 transports prostaglandins. An analysis of the three-dimensional protein structure affected by the nsSNP (AX-168186923) showed that the amino acid changed from Methionine to Valine, but the overall protein structure remained unchanged. Conclusions: The SNPs selected in this study can be used to identify genetic factors, associated with pyometra and vulnerable breeds. This is expected to reduce companion animal related costs and the psychological burden on owners.
4,200원
8.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Cryopreserved semen and embryos are essential tools in livestock reproduction, enabling genetic improvement and herd management. Although these materials are theoretically stable in liquid nitrogen (LN2), viability often decreases over time, particularly in farm settings. Micro-ice crystals (MICs) are hypothesized to form under poor LN2 handling conditions, potentially compromising the survival of frozen genetic resources. However, the extent and impact of MIC accumulation have not been thoroughly quantified. Methods: This study evaluated MIC accumulation and its effects on the viability of cryopreserved bovine semen and embryos under different LN2 storage environments and conditions. MIC content was measured by filtering 10 L of LN2 through nonwoven fabric and weighing the retained crystals and debris. The viability of sperm and embryos were assessed using a computer-assisted semen analysis (CASA) and blastocoel re-expansion. Results: MIC content was 3.5 times higher in farm-stored LN2 than in laboratory LN2, with significantly more debris also detected. Progressive motility and velocity parameters (VCL, VAP, VSL) were similarly reduced. Blastocyst survival dropped significantly under farm conditions after six months (42.4%) compared to laboratory storage (84.4%, p < 0.05). These findings suggest a strong correlation between MIC accumulation and decreased post-thaw viability of cryopreserved materials. Conclusions: MICs formed in LN2 due to environmental exposure and poor handling can severely impair the viability of cryopreserved sperm and embryos. Regular filtration and improved LN2 management, especially in farm environments, are essential to reduce MIC-related damage. These practices may enhance the long-term usability and reliability of genetic resources in livestock breeding programs.
4,000원
9.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Cigarette smoke is a well-known reproductive toxicant that causes structural and functional damage to the testis through oxidative stress and apoptotic pathways. This study aimed to evaluate whether apple seed extract (ASE) can mitigate testicular damage induced by tobacco smoke extract (TSE) in a mouse model. Methods: Adult male mice were orally administered TSE to induce testicular injury. ASE was delivered intraperitoneally, either as a post-treatment or concurrently with TSE. Histological evaluation (H&E staining), immunohistochemistry (IGF-1 and VEGF), and immunofluorescence (TNF-α, Caspase-3, and BCL-2) were conducted to assess tissue morphology, growth factor expression, and apoptotic signaling. Results: TSE exposure led to degeneration of the seminiferous tubules, suppressed IGF-1 and VEGF expression, and increased pro-apoptotic markers (TNF-α, Caspase-3), along with reduced BCL-2 expression. ASE treatment restored seminiferous tubule architecture, enhanced the expression of growth and anti-apoptotic factors, and attenuated apoptotic signals. These restorative effects were particularly significant in the post-treatment groups. Conclusions: ASE demonstrated protective and reparative effects against TSE-induced testicular toxicity by modulating oxidative and apoptotic pathways. These findings highlight ASE as a promising natural therapeutic candidate for ameliorating smokerelated male reproductive dysfunction.
4,300원
10.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: The preservation of boar semen is essential for optimizing artificial insemination outcomes and maintaining sperm functionality during liquid storage. Glucuronic acid (GA) is involved in cellular detoxification and homeostasis regulation and may relate to oxidative processes. However, its effect on boar semen preservation remains unclear. This study aimed to investigate the effects of GA addition on sperm viability, acrosome reaction, and intracellular ROS level during liquid storage in pigs. Methods: Boar semen was collected by the gloved-hand method and transported at 17℃. Samples were diluted with extenders containing 0, 0.5, 1.0, 2.5, and 5.0 mM GA and stored at 17℃ for 7 days. Sperm viability, acrosome reaction, and intracellular ROS levels were detected using SYBR-14/PI, FITC-PNA/PI, and DCFDA/PI double staining and analyzed by flow cytometry. Results: Sperm viability was higher in the 0.5 mM GA group than in the 0 mM group at 72 h, with no difference at 120 h and 168 h. Acrosome reaction showed no difference at 72 h and 120 h, but was lower in the 5.0 mM group than in the 0 mM group at 168 h. Intracellular ROS levels were similar among treatments at 72 h and 168 h, but were higher in the 5.0 mM group than in the 0 mM group at 120 h. Conclusions: GA supplementation showed concentration- and storage-dependent effects, with a transient viability increase at 0.5 mM (72 h) and a reduced proportion of acrosome-reacted sperm at 5.0 mM (168 h), while ROS was not decreased and was elevated at 5.0 mM at 120 h, suggesting the need for dose optimization.
4,000원
11.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Mitoquinone mesylate (MitoQ), a synthetic analog of coenzyme Q10 with antioxidant properties, has demonstrated significant improvements in mitochondrial penetration compared to coenzyme Q10, demonstrating potential medicinal benefits. We investigated the effects of MitoQ on neonatal testicular development using ex vivo mouse testicular fragments (MTFs). Methods: Testicular cell differentiation was assessed by culturing MTF from 5-day-old male mice in vitro with 0.01-1 μM MitoQ for 5 days. Cell differentiation, proliferation, and distribution within the testes were assessed using immunohistochemical staining and analysis of gene and protein expression. Results: Histological analysis revealed that 0.01-1 μM MitoQ induced germ cell miogenesis in the seminiferous tubules, increasing the number of Sycp3-positive cells and reducing the genetic expression of the undifferentiated marker Sall4. Sertoli and Leydig cell markers increased at the genetic and protein levels, as confirmed by immunohistochemical staining. In addition, MitoQ increased the expression of antioxidant regulatory and antioxidant enzyme-related factors. Conclusions: Overall, MitoQ can induce the expression of antioxidant factors in in vitro cultured neonatal testes while simultaneously enhancing germ cell differentiation, Sertoli cell proliferation, and Leydig cell maturation and proliferation.
4,000원
12.
2025.12
구독 인증기관 무료, 개인회원 유료
Background: Endothelial cells (EC) that make up the inner wall of blood vessels play an important role in angiogenesis and vascular recovery. Cardiovascular disease caused by dysfunction of ECs has been reported as a major cause of death worldwide. Despite significant research so far, the underlying mechanism of dysfunction of ECs in cardiovascular disease progression is not yet fully understood. Although therapeutic transplantation of autologous ECs is limited due to lack of cell availability, adiposederived stem cells (ADSCs), known for their ease of procurement and high potential for differentiation, will provide promising solutions to generate autologous ECs. Methods: This study investigated the optimal differentiation of ADSCs into ECs under EBM-2 culture conditions supplemented with VEGF and BMP-4 in hypoxia (2% O2). Results: During 14 days of in vitro differentiation, cells cultured in EBM-2 supplemented with VEGF showed the characteristics of early vascular ECs and some cells adopted polygonal forms. Conversely, cells cultured in EBM-2 and hypoxia supplemented with both VEGF and BMP-4 differentiated into the typical cobblestone morphology that appears in vascular ECs. As a result of immunostaining against the vascular ECs marker CD-31, CD-31 expression was increased under EBM-2 culture conditions with VEGF and VEGF + BMP-4 in hypoxia, but expression was insufficient in normal oxygenation (21% O2). In the flow cytometry analysis, high expression of CD-31 expression was observed under conditions including both VEGF and BMP-4 of hypoxia. Interestingly, in gene expression, the pluripotency marker OCT-3/4 was significantly reduced under hypoxic conditions, but SOX2 and NANOG expression were higher than under normal oxygen conditions. However, CD-31 expression was significantly higher under differentiation conditions in which VEGF and BMP-4 were added under hypoxia conditions. In a functional analysis, CD-31-positive ADSC-derived ECs differentiated under hypoxia had excellent tube formation and Dil-Ac-LDL uptake, which are important for vascular repair and function. Conclusions: These findings confirmed the therapeutic usefulness of ECs derived from ADSC for the treatment of cardiovascular disease due to the synergy effect of hypoxia and BMP-4.
4,600원
