간행물
한국동물생명공학회지 (구 한국수정란이식학회지) KCI 등재 Journal of Animal Reproduciton and Biotechnology
- 발행기관 한국동물생명공학회(구 한국수정란이식학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2671-4639 (Print)2671-4663 (Online)
- 수록기간 1983 ~ 2026
- 주제분류 자연과학 > 생물공학 자연과학 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Vol.41 No.1 (2026년 3월) 5건
1.
2026.03
구독 인증기관 무료, 개인회원 유료
Diego Gouvêa de Souza, Fernanda Nunes Marqui, Sabrina Cruz Tfaile Frasnelli, Andressa Vilas Boas Nogueira, Alicio Martins Jr., Ana Carolina Monteirinho Lobo, Claudio Willian de Oliveira Delfes-Camargo, Luan Sitó-Silva, Eunice Oba
Background: When chemically defined media are used for in vitro maturation (IVM), fetal calf serum (FCS) and bovine serum albumin (BSA) are often replaced with synthetic macromolecules such as polyvinyl alcohol (PVA). However, the developmental competence of oocytes under these conditions is typically reduced, with lower blastocyst formation rates. This study aimed to quantify the transcripts of GDF9, BMP15, and OOSP1 in oocytes, and GREM1, PTGS2, PFKP, AREG, EREG, HAS2, VCAN, PTX3, ADAM10, and ADAM17 in cumulus cells. Methods: Oocytes were divided into three groups: immature oocytes at the germinal vesicle stage (GV), in vivo matured oocytes (IVMO), and oocytes matured in vitro in IVM medium supplemented with 10% FCS, 4 mg/mL BSA, or 1 mg/mL PVA. For the IVMO group, ten donor cows were superovulated with follicle-stimulating hormone (FSH), and cumulus–oocyte complexes (COCs) were recovered by ovum pick-up (OPU) 19-20 hours after gonadorelin administration. Gene expression was evaluated in oocytes and cumulus cells using quantitative real-time PCR. Results: The relative transcript levels of GREM1, PTGS2, PFKP , and AREG were significantly higher (p < 0.05) in cumulus cells from IVMO compared with those from GV or IVM oocytes. Additionally, oocytes matured in vitro in medium supplemented with FCS showed increased (p < 0.05) expression of GREM1 and AREG compared with those cultured with BSA or PVA. Conclusions: FCS supplementation during IVM positively influenced the transcription of GREM1 and AREG. However, the superior expression profile observed in cumulus cells from in vivo matured oocytes highlights the need for improved IVM media and culture conditions to enhance oocyte competence.
4,200원
2.
2026.03
구독 인증기관 무료, 개인회원 유료
Background: Cryptorchidism is the most common congenital abnormality affecting male reproductive development in dogs. Castration is generally recommended for affected individuals, and many cases are identified incidentally during elective procedures. A better understanding of the epidemiology and clinical characteristics of cryptorchidism may enhance awareness among veterinarians and dog owners, thereby contributing to improved surgical management and postoperative care. Methods: Clinical data were retrospectively collected from dogs diagnosed with cryptorchidism between 2018 and 2025. Cryptorchidism was classified according to the anatomical location and laterality of the undescended testis. Breed-related associations were evaluated by comparing distribution patterns between purebred and mixed-breed dogs using Fisher’s exact test and odds ratios analyses. Results: Among 618 dogs undergoing castration, 80 were diagnosed with cryptorchidism (12.94%). Right-sided unilateral cryptorchidism was the most common presentation (n = 55, 8.90%), followed by left-sided unilateral cryptorchidism (n = 17, 2.75%), and bilateral cryptorchidism was the least frequent (n = 8, 1.29%). Undescended testes were more frequently located in the inguinal region (n = 53, 66.25%) than in the abdominal cavity (n = 27, 33.75%). No statistically significant breed-specific differences in anatomical location were detected when compared with mixed-breed dogs. Conclusions: This study characterizes dog cryptorchidism based on anatomical location and laterality, providing baseline clinical data on breed-related distribution patterns that may support future studies of clinical decision-making.
4,000원
3.
2026.03
구독 인증기관 무료, 개인회원 유료
Background: The rise in annual mean temperatures caused by global warming has increased the risk of heat stress in livestock. Dairy cows are particularly susceptible, with both productivity and reproductive performance adversely affected. Previous studies have reported that THI ≥ 72 is generally considered the onset of heat stress. Against this background, the present study examines conception rate variations of dairy cows in relation to the THI in Jeolla-do region through 2024. Methods: The THI for the Jeolla-do region was collected from the Korea Meteorological Administration Data Portal, and artificial insemination conception records were compiled for 35,053 cows from 39 farms across Gwangju Metropolitan City and eight counties in Jeolla-do region (Gochang, Gwangju, Yeonggwang, Naju, Hampyeong, Muan, Jangseong, and Yeongam). Seasonal artificial insemination conception rates (spring: March-May, summer: June-August, autumn: September-November, and winter: December-February) were compared and analyzed in relation to the regional THI values (n = 35,053). Results: The THI threshold for heat stress in dairy cows (THI ≥ 72) persisted from May through September, and all locations, except Yeongam and Naju, reached their annual maximum THI in August. In Jangseong, THI remained ≥ 72 for the longest period, lasting 175 days. In most locations, the highest monthly conception rate occurred in July. By season, conception rates were highest in summer (June-August) at 58.5% and lowest in autumn (September-November) at 43.5%. Conclusions: The pronounced reduction in conception rates observed in autumn appears to be a consequence of the cumulative heat stress of the summer, which continues to impair reproductive performance even in autumn. Although farms commonly deploy cooling systems such as ventilation fans and sprinklers during the summer, management of heat stress may be neglected in autumn despite animals continuing to experience thermal stress, thus contributing to the observed decline in conception rates. These findings underscore the need for vigilant herd management and heat‑stress mitigation not only in summer but also in autumn. The results can serve as baseline data for future research on the effects of heat stress on dairy cattle and on strategies for its mitigation.
4,000원
4.
2026.03
구독 인증기관 무료, 개인회원 유료
Background: Myostatin (MSTN) gene is a major inhibitor of skeletal muscle growth, and variants within its promoter region may affect transcriptional activity and musclerelated traits. Mongolian native horses have adapted to endurance and harsh environmental conditions, whereas Halla horses exhibit enhanced musculature owing to crossbreeding with Thoroughbreds. MSTN promoter variations in these breeds have not been sufficiently investigated, highlighting a critical research gap that motivated the present study. Methods: Two single nucleotide polymorphisms (SNPs) in the MSTN promoter region were examined in 19 Mongolian and 4 Halla horses using PCR amplification and Sanger sequencing. Additionally, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway annotations were used to characterize gene functions. Results: Two promoter region SNPs were identified in MSTN gene. These distribution patterns represent preliminary observations within the sampled populations, providing a valuable foundation for future investigations. Functional annotation further supported the regulatory relevance of MSTN through its involvement in muscle growth inhibition and the TGF-β/ACVR2B/SMAD pathway. Conclusions: This study provides the first baseline characterization of MSTN promoter polymorphisms in Mongolian and Halla horses. The g.26T>C variant was identified in Mongolian horses, whereas the shared g.156T>C SNP represents a regulatory variant observed in both breeds, pending confirmation in broader populations. However, due to the limited sample size, we consider these findings to be preliminary observations rather than definitive evidence of breed-specific adaptation. The results of this study are expected to serve as fundamental data for establishing future genetic information– based breeding and conservation strategies for Mongolian horses.
4,000원
5.
2026.03
구독 인증기관 무료, 개인회원 유료
Background: The morphological characteristics of sperm are essential for assessing sperm quality, which partially influences reproductive efficiency variables such as litter size in sows. Recently, deep-learning-based object detection algorithms have been explored to detect and classify sperm morphological features, with the training of these models requiring sperm microscopy image data. The performance of these models in detecting morphological features was significantly affected by the size of the dataset and the image quality of the images. Methods: This study proposed a deep-learning-based super-resolution (SR) algorithm to enhance the quality of sperm microscope images. The model was trained using a dataset consisting of high-resolution (HR) original and low-resolution (LR) images generated by downscaling the original images through bicubic interpolation. The SR results of the test dataset were compared with those of the original HR images to evaluate their performances using the peak signal-to-noise ratio (PSNR) and structural similarity index measure (SSIM). Statistical analyses were conducted to examine the performance differences based on the training models and dataset types. Results: This study indicated that the SR images showed no significant differences in PSNR (p = 0.9740) and SSIM (p = 0.9864) compared with the original HR images in same magnification. Moreover, increased processing speed was observed with reductions in model hyperparameters. While processing SR images improved spatial resolution across various microscopic magnifications, the overall image quality did not exceed that of the original HR images. Conclusions: SR models applied to sperm microscopy images outperformed conventional SR algorithms. These findings suggest that SR algorithms hold promise for improving the quality of LR microscopic images in future deep-learning-based object-detection algorithms.
4,000원
