Vibrio parahaemolyticus (V. parahaemolyticus) has been recognized as a significant food-borne pathogen around the world. In this study, we investigated the prevalence and antimicrobial resistance of V. parahaemolyticus isolated from raw fishes. A total of 64 samples of raw fishes purchased from a traditional seafood market in Seoul, Korea. were examined for the presence of V. parahaemolyticus using intestines, gills, and fins. Twenty five grams of all samples were enriched in 225ml of alkaline peptone water at 37℃ for 24h and then streaked onto thiosulfate citrate bile sucrose agar. Suspected colonies were inoculated into triple sugar iron agar for biochemical screening test and were finally confirmed with API 20NE strip. Antimicrobial resistance tests were performed with disc diffusion method in accordance with National Committee for Clinical Laboratory Standard. Thirty three V. parahaemolyticus strains were isolated from raw fishes among 33 out of 64 (51.6%). Among 33 isolates, 16 isolates (48.5%) were resistant to ampicillin, 7 isolates (21.2%) were resistant to amikacin, and all isolates were not resist to other antibiotics such as amoxicillin & clavulanic acid, sulfamethoxazole & trimethopenem, ciprofloxacin, cefotaxime and cefepime. Although the prevalence of V. parahaemolyticus was high in raw fishes compared to other studies, antimicrobial resistance rate of the isolates was relatively low. These results could be useful information for risk assessment of V. parahaemolyticus in raw fishes.

ABSTRACT
 서 론
 재료 및 방법
  1. 실험에 사용된 시료
  2. V. parahaemolyticus의 분리 및 동정
  3. 항생제 감수성 시험
 결과 및 고찰
  1. V. parahaemolyticus의 분리율
  2. 분리된 균주의 항생제 내성 양상
 참고문헌

• 성창현(건국대학교 수의과대학) | Chang-Hyeon Sung (Department of Public Health, College of Veterinary Medicine, Konkuk University)
• 천정환(건국대학교 수의과대학) | Jeong-Hwan Cheon (Department of Public Health, College of Veterinary Medicine, Konkuk University)
• 현지연(건국대학교 수의과대학) | Ji-Yeon Hyeon (Department of Public Health, College of Veterinary Medicine, Konkuk University)
• 황인균(식품의약품안정청) | In-Gyun Hwang (Food Evaluation Department, Food Microbiology Division, Korea Food and Drug Administration)
• 곽효선(식품의약품안정청) | Hyo-Sun Kwak (Food Evaluation Department, Food Microbiology Division, Korea Food and Drug Administration)
• 윤상현(식품의약품안정청) | Sang-Hyeon Yoon (Food Evaluation Department, Food Microbiology Division, Korea Food and Drug Administration)
• 이정수(식품의약품안정청) | Jeong-Soo Lee (Food Evaluation Department, Food Microbiology Division, Korea Food and Drug Administration)
• 정윤희(한국소비자원) | Yun-Hee Chung (Korea Consumer Ageny)
• 송광영(건국대학교 수의과대학) | Kwang-Young Song (Department of Public Health, College of Veterinary Medicine, Konkuk University)
• 서건호(건국대학교 수의과대학) | Kun-Ho Seo (Department of Public Health, College of Veterinary Medicine, Konkuk University) Correspondence