우리나라 여러 해양환경 지역으로부터 확보한 370주의 해양세균, 균류, 미세조류로부터 기초생 리활성(항산화, 항염, 항균, 항암, 항바이러스)을 조사하여 채집지, 분리원, 종(種) 수준에서의 활성결과를 비교하였다. 해양세균의 경우, 일반적으로 유용성이 많이 알려진 Streptomyces 속 과 Bacillus 속에 속하는 균주들이 두드러진 강한 효능이 관찰되었고, 주로 해양퇴적물로부터 유용한 자원을 분리할 수 있었다. 해양균류와 미세조류의 경우에도 종 특이적으로 활성이 강 하게 나타나는 결과를 확인할 수 있었고, 효능 특이적으로 활성을 보이는 결과도 얻을 수 있었 다. 이러한 결과를 바탕으로 추후 특정질병에 선택적으로 효능을 보이는 화학물질 연구 또는 자원 기반 연구 수행 시 유용성을 전제로 한 자원 확보 전략 수립과 산업 활성화를 위한 전 략소재로 우선적 접근이 용이할 수 있는 연구결과라 생각된다. 또한, 이들 결과를 해양바이오 뱅크를 통한 분양소재로 활용함으로써 학계, 산업계에서 활용하여 해양바이오산업 활성화에 좀 더 빠른 접근을 도울 수 있다고 생각한다.
본 연구는 K-뷰티브랜드의 CSR동기가 CSR 진정성과 브랜드 신뢰에 주는 영향과 CSR 진정성 과 브랜드 신뢰가 브랜드 지지에 주는 영향을 확인하고자 한·중 소비자 데이터를 이용해 비교연구하여 진 행하였다. 수집된 데이터 중 392부를 검증에 사용하였고 2단계 접근법과 최우도 측정법을 활용해 분석하였 다. 연구 결과, 자기본위적 동기를 제외한 가치지향적, 이해관계자지향, 전략적 동기는 CSR 진정성과 브랜 드 신뢰에 긍정적 영향을 주는 것으로 나타났다. 또한 CSR 진정성은 브랜드 신뢰에 긍정적 영향을 주었고, 이는 브랜드를 지지하는 것으로 나타났다. 한·중 소비자를 비교한 결과, 두 나라 모두 CSR동기가 진정성과 브랜드 신뢰에 영향을 주는 것으로 나타났으나 중국소비자가 한국소비자보다 더 많은 부분에서 높게 나타 난 결과를 보였는데 이러한 결과는 K-뷰티 브랜드의 중국시장 확장을 위해서는 중국소비자를 이해하고 그 들이 추구하는 CSR동기 전략을 강화해야 한다는 시사점을 가진다.
This study evaluated the quality of school milk programs and analyzed the relative importance of school milk program selection attributes using conjoint analysis. The survey was conducted on students from middle and high schools in metropolitan cities that provide school milk programs. Responses were received from 414 students and the data was subjected to frequency analysis, t-test, and conjoint analysis using the SPSS Statistics Package. While evaluating white milk in the school milk program, middle school students rated ‘packaging condition’ (4.23) the highest, high school students rated ‘nutrition’ (4.64) the highest, and their evaluation of all the quality attributes was significantly different from that of middle school students (p<0.001). Overall satisfaction scores too, showed a significant difference between high school (4.46) and middle school students (4.01) (p<0.001). Processed milk & dairy products had the highest satisfaction score in the attribute of ‘serving time’ (4.57). The relative importance of the choice attributes of the school milk program was in the order of ‘number per item’ (62.260%), ‘temperature’ (25.708%), and ‘serving method’ (12.032%) for all students. The school milk program most preferred by all students and middle school students was to provide milk at a refrigerated temperature, select white milk three times a week, processed milk, fermented milk, and cheese twice a week, and provide it at the desired time.
순수 베타 핵종인 90Sr 분석은 화학적 거동이 유사한 알칼리 토금속(Ca, Ba, Ra)등 방해 원소를 제거할 분리 공정이 필요하다. 본 연구는 Sr을 추출/정제하기 위해 추출크로마토그래피법을 이용한 최적의 절차를 마련하는 것을 목적으로 한다. Sr resin 1.5 mL BV(Bed Volume)의 최대 Sr 흡착량은 6 mg이었다. 유량 1 mL min-1과 Ca 200 mg 이하에서는 Sr resin 1.5 mL(BV) 의 Sr 회수는 정량적이었으나, 유량을 5 mL min-1으로 중가 시키면 Sr 회수율이 감소하였다. 같은 양(BV)의 Sr resin을 사용 할 경우, 컬럼의 단면적이 작을수록 Sr resin의 분리능이 향상되었다.
The purposes of this study were to identify the relative importance attributes, deduct the ideal combination of total utility and establish the marketing strategies for quality improvement of foodservice at funeral halls of medical centers. Data were collected using self-administered questionnaires from 102 funeral foodservice employees and 71 chief mourners or the bereaved. According to the results from a conjoint analysis, among foodservice employees at funeral hall, the relatively important attributes were 'taste (52.84%)', 'menu variety (24.419%)' and 'price (22.741%)'; among chief mourners or the bereaved, they were 'taste (50.004%)', 'price (31.388%)' and 'menu variety (18.008%)'. The ideal combination of total utility was different between funeral foodservice employees and chief mourners or the bereaved; it was higher among chief mourners or the bereaved (1.211) compared to funeral foodservice employees (1.169). Thus, there should an endeavor to improve the foodservice quality in funeral halls of medical centers through better taste, low price and similar menu variety.
Vibrio parahaemolyticus (V. parahaemolyticus) has been recognized as a significant food-borne pathogen around the world. In this study, we investigated the prevalence and antimicrobial resistance of V. parahaemolyticus isolated from raw fishes. A total of 64 samples of raw fishes purchased from a traditional seafood market in Seoul, Korea. were examined for the presence of V. parahaemolyticus using intestines, gills, and fins. Twenty five grams of all samples were enriched in 225ml of alkaline peptone water at 37℃ for 24h and then streaked onto thiosulfate citrate bile sucrose agar. Suspected colonies were inoculated into triple sugar iron agar for biochemical screening test and were finally confirmed with API 20NE strip. Antimicrobial resistance tests were performed with disc diffusion method in accordance with National Committee for Clinical Laboratory Standard. Thirty three V. parahaemolyticus strains were isolated from raw fishes among 33 out of 64 (51.6%). Among 33 isolates, 16 isolates (48.5%) were resistant to ampicillin, 7 isolates (21.2%) were resistant to amikacin, and all isolates were not resist to other antibiotics such as amoxicillin & clavulanic acid, sulfamethoxazole & trimethopenem, ciprofloxacin, cefotaxime and cefepime. Although the prevalence of V. parahaemolyticus was high in raw fishes compared to other studies, antimicrobial resistance rate of the isolates was relatively low. These results could be useful information for risk assessment of V. parahaemolyticus in raw fishes.
Recently there has been an increasing amount of foreign livestock products distributed in the domestic market due to the market opening. Some vicious dealers sell the foreign beef in the trade name of the native beef during the final distribution step to arouse the social criticism frequently. In this report, we investigated a method to distinguish the native beef from the foreign one scientifically using the PCR-RAPD, a recent gene technique. Hygienical safety was also examined using a microbiological test for toxicity of Escherichia coli O157:H7 and the food poisoning bacteria. The conditions of DNA amplification for the PCR analysis were 1 × Taq polymerise buffer, 1.5 mM MgCl₂, 50 uM dNTP, 100 ng primers, 2.5 unit Taq polymerise and 5-20 ng template DNA, with the final volume of 50 Etl. The size of the amplified product was detected mostly in the range of 0.5-2.0 kbp. The size of DNA, gene marking factor, which could be a criterion distinguishing the native beef from the foreign one, appeared approximately 1.2 kbp. The native beef was distinguished from the foreign beef with more than 90% of confidence by the gene marking factor. This method was expected to be useful in the breed discrimination between the native beef and the foreign one. The hygienical test results showed that, fortunately, neither Salmonella spp. and Listeria monocytogenes which form a principal cause of the food poisoning nor Enterohemorrhagic Escherichia coli : EHEC which have provoked a recent social disturbance, were detected at all.
참기름의 이종기름 함유 유무를 판단하기 위한 기초자료를 얻고자 국내산 참기름의 옥배유를 혼합한 시료와 중국산 참기름에 대하여 지방산, sesamolin 및 sesamin 함량, AOM에 의한 유도기간을 각각 조사했다. 국내산 참기름에 옥배유를 혼합할 경우, stearic acid는 옥배유를 20% 이상 혼입한 참기름에서 국내산 참기름과 뚜렷한 차이를 나타내었다. 그리고 Oleic acid와 linoleic acid에서는 10%의 혼합시에도 미약하나마 함량의 차이를 보였다. 참기름에 옥배유를 혼합하 fruddn stearic/linoleic 비율은 옥배유의 혼입된 함량과 비례하였다. 국내산 참기름에 이종기름으로 drhqodb를 30% 혼합시에는 sesamolin이 18.3%, sesamin이 21.0% 감소하였다. AOM에 의한 유도 기간은 중국산, 백배유 20% sesamolin과 sesamin의 함량 및 AOM에 의한 유도기간 측정 등이 적용하리라 생각된다.
Microbial distribution as well as content of salt and preservatives in side dishes was investigated by analyzing cell count of viable cells, coliform bacteria and food poisoning bacteria of sliced squid bokum and anchovy bokum, purchased at 17 dift'erent department stores and 2 different traditional markets in Seoul, which are most preferred by many consumers to any other side dishes available in the market. 6.2 × 10³-1.2 × 10^5 cells/g of viable cells was detected in 19 different samples of the sliced squid bokum, among which samples collected at the traditional markets counted the highest number of viable cells. 16 samples of the sliced squid bokum and 14 samples of the anchovy bokum contained 10³-10^8 cells/g of coliform group. However food poisoning bacteria were not detected in all the samples tested. Salt content was 2.42-4.89 %w/w and 2.28-6.46 %w/w for the sliced squid bokum and the anchovy bokum respectively. Analysis of preservatives by HPLC such as sorbic acid, benzoic acid, dehydroacetic acid and another 6 kinds of esters resulted that 1.0× 73.8 mg/100 g of sorbic acid was detected in the 19 samples of the sliced squid bokum, while only 6 samples of the 15 anchovy bokum samples contained sorbic acid.