A confirmatory method based on liquid chromatography with tandem mass spectrometry was developed for determination of 12 aminoglycosides in milk. Extraction of aminoglycosides from milk was performed using by liquid extraction using a 10 mM phosphate buffer containing 2% (w/v) trichloroacetic acid, followed by performance of a solid-phase clean-up procedure on a hydrophilic-lipophilic balance solid-phase extraction (HLB SPE). Ion-pair chromatography, using a mixture of 20 mM heptafluorobutyric acid (HFBA) in water and acetonitrile as the mobile phase, was used for retention of aminoglycosides on a reversed-phase C18 column. Mass spectral acquisition was performed in the multiple reaction monitoring mode, selecting two precursor ion>product ion transitions for each target compound. Satisfied recoveries (70.1～109.6%) of all aminoglycosides were demonstrated in spiked milk at three levels from 50 ng/g to 200 ng/g. The coefficients of variation ranged from 3.2% to 14.0%. The limits of quantitation (LOQs) for aminoglycosides ranged from 2.5 ng/g to 40.3 ng/g.

ABSTRACT
 서 론
 재료 및 방법
  1. 표준품 및 시약
  2. 분석기기 및 분석조건
  3. 표준용액 조제
  4. 표준검량선 작성
  5. 시료 전처리법
  6. 분석법 검증
 결과 및 고찰
  1. LC-MS/MS 분석조건 확립
  2. 시료 전처리법 확립
  3. 분석법 검증
 참고문헌

• Chae-Mi Lim(Animal, Plant and Fisheries Quarantine and Inspection Agency) Correspondence
• Byung-Hoon Cho(Animal, Plant and Fisheries Quarantine and Inspection Agency)
• Gap-Su Chung(Animal, Plant and Fisheries Quarantine and Inspection Agency)
• Seong-Wan Son(Animal, Plant and Fisheries Quarantine and Inspection Agency)