Purpose: This study examined the effects of α-lipoic acid in diluted solvents on cell growth in 3T3-L1 cells according to the treated concentration and times. Methods: Adipocyte 3T3-L1 cell were cultured. Confluent cells underwent starvation with SFM for 1 day and then were cultured in a medium containing various concentrations 0, 100, 200, and 400 μmol/L of α-lipoic acid. The cell viability was measured using the EZ Cytox assay kit. In addition, the effect of α-lipoic acid of diluted solvents on the cell growth in 3T3-L1cells was examined according to the treated concentration and times. Results: The α- lipoic acid diluted ethanol inhibited cell proliferation in a dose and time dependent manner. The α-lipoic acid diluted ethanol induced adipocyte 3T3-L1 cells proliferation with an adipocyte inducer. In addition, α-lipoic acid inhibited adipocyte 3T3- L1 growth in a dose and time dependent manner (p<0.05). Conclusion: This study showed that a treatment with α-lipoic acid diluted ethanol inhibits cell growth of, adipocyte 3T3-L1 cells induced with an adipocyte inducer, (200 μmol/L of α- lipoic acid) treated for 48 hr.

Abstract
 I. 서 론
 II. 연구내용 및 방법
  1. 실험재료 및 세포 배양
  2. 지방세포분화 유도
  3. 세포 성장
  4. 통계 처리
 III. 결과 및 고찰
  1. α-Lipoic acid의 희석 용매별 세포 증식
  2. α-Lipoic acid의 처리 농도에 따른 세포 증식
  3. α-Lipoic acid의 처리 시간에 따른 세포 증식
 V. 요약 및 결론
 References

• 서은영(장안대학교 식품영양과) | Eunyoung Seo (Department of Food and Nutrition, Jangan University) Corresponding author