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Background : The health-promoting effects of green tea are mainly attributed to its polyphenol content, particularly flavanols and flavonols, which account for 30% of a fresh leaf’s dry weight, but the ingredients of the polyphenol content vary depending on the species. This study was conducted to select some candidates with superior anti-oxidative and anti-allergy effects from among seven cultivars of green tea.
Methods and Results : Green tea extracts were prepared by extraction with ethanol and by evaporation of the solvent at low pressure. To evaluate their anti-allergy effect and cell viability, the samples were tested for ß-hexosaminidase inhibitory activity and MTT assay of the RBL-2H3 cells, respectively. The anti-oxidation effects of the samples were analyzed with a DPPH radical scavenging activity. According to the results of the experiment, four extracts including Camelia sinensis var. Kemsull, C. sinensis var. Beachwisull, C. sinensis var. Chamnok and C. sinensis var. Fushun showed effective ß-hexosaminidase inhibitory activity at 12.5, 25, 50, 100 ㎍/㎖. At 50 ㎍/㎖, C. sinensis var. Saemidori had the highest cell viability as 86.1%, and all of the samples showed cell proliferation above 70% at 25 ㎍/㎖. The extract of C. sinensis var. Kemsull showed a 60 - 70% inhibitory effect on the DPPH radical at all of the tested concentrations, whereas the extracts of C. sinensis var. Ryohu, C. sinensis var. Saemidori, C. sinensis var. Yabukita showed lower DPPH inhibition effects at around 10 - 30%.
Conclusion : The results of this study indicate that the extracts of C. sinensis var. Kemsull, C. sinensis var. Beachwisull, and C. sinensis var. Chamnok have more prominent anti-oxidation and anti-allergy effects than other cultivars, and thus could be utilized as resources for improving health.
