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남아시아 수집 홍화자원의 세로토닌 유도체 함량 분석

Analysis of Serotonin Derivatives in Safflower Germplasm Collected from South Asia

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/362913
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : To select potential plant resources as natural antioxidants and functional materials, e valuation of N-(p-coumaroyl)serotonin (CS) and N-feruloylserotonin (FS) in 43 safflower accessions collected from South Asia was conducted.
Method and Results : CS and FS were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total phenolic content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis-3- ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities, ferric reducing antioxidant power (FRAP) and reducing power (RP). The mean CS content was 32.74 ㎎/g dried extract (DE) with a range from 3.44 to 83.30 ㎎/g DE, and the FS content ranged from 1.43 to 34.53 ㎎/g·DE with a mean of 12.69 ㎎/g DE. The mean of TPC of 43 safflower accessions was 55.22 ㎍ GAE/㎎·DE. The average values of antioxidant activities based on DPPH, ABTS, FRAP, and RP assay showed 48.77 ㎍ ASC eq/㎎ DE, 97.62 ㎍ ASC eq/㎎ DE, 70.22 ㎍ ASC eq/㎎ DE, and 50.01 ㎍ ASC eq/㎎ DE, respectively. The 43 safflower accessions were classified into two groups based on the complete linkage cluster analysis using their CS, FS, TPC, DPPH, ABTS, FRAP and RP. Group Ⅱ showed higher CS, FS and antioxidant activity than Group Ⅰ (p < 0.05). K185245 and K185247 were included in Group Ⅱ, K185245 had the highest CS and FS, and K185247 was the highest in TPC, DPPH, and ABTS. CS had significant positive correlation with FS (r = 0.849**). Significantly high correlation coefficients were recorded between TPC and antioxidant assays including DPPH, ABTS, and RP. The first two principal components had accounted for the 80.46 % of the total variance.
Conclusion : These results showed that K185245 and K185247 could be used as a source of valuable natural antioxidant materials and could be useful to develop new functional materials.

저자
  • 정이진(농촌진흥청 국립농업과학원 농업유전자원센터) | Yi Jin Jeong
  • 김다정(농촌진흥청 국립농업과학원 농업유전자원센터) | Da Jeong Kim
  • Awraris Derbie Assefa(농촌진흥청 국립농업과학원 농업유전자원센터)
  • 허온숙(농촌진흥청 국립농업과학원 농업유전자원센터) | On Sook Hur
  • 노나영(농촌진흥청 국립농업과학원 농업유전자원센터) | Na Young Ro
  • 이재은(농촌진흥청 국립농업과학원 농업유전자원센터) | Jae Eun Lee
  • 이호선(농촌진흥청 국립농업과학원 농업유전자원센터) | Ho Sun Lee
  • 이주희(농촌진흥청 국립농업과학원 농업유전자원센터) | Ju Hee Rhee
  • 성정숙(농촌진흥청 국립농업과학원 농업유전자원센터) | Jung Sook Sung Corresponding author