Promalactis established by Meyrick in 1908, is one of the highest species richness genera of the family Oecophoridae. Over 240 described species are mainly distributed from Oriental and Palaearctic regions. They are usually a tiny-sized and exhibit subtle morphological differences which make problems on morphological-based identification. In this study, we performed DNA barcoding study for the Promalactis by using mitochondrial cytochrome c oxidase subunit I (COI). From analyzing 155 COI sequences, we observed the usefulness of the COI in species identification with intraspecific genetic variation (range 0.0-2.1%) and interspecific genetic divergence (range 2.8-15.4%).