This study was divided to three experiments for evaluating the effects of pre- or post-load 1-MCP on quality of ‘Fuji’ apples exposed to ethylene. The first experiment was compared for fruit quality at room temperature at 7, 14, 21, and 28 days between the control and 1-MCP-treated fruits followed storage of 130, 150, and 170 days. 1-MCP-treated fruits maintained higher fruit titratable acidity and firmness than those of control fruits. The second experiment was compared for fruit quality at room temperature at 7, 14, 21, and 28 days between fruits applied with pre-loaded-1-MCP on ethylene treatments (10 μL/L, 20 μL/L, and 40 μL/L) and control fruits. 1-MCP-treated fruits were not affected by ethylene concentrations, and had higher fruit weight and firmness than those of control. The third experiment was compared for fruit quality at room temperature at 7, 14, 21, and 28 days between fruits applied with pre-loading with ethylene (10 μL/L) followed by ventilation (8 h, or 32 h, or 56 h) and 1.0 μL/L 1-MCP treatment. As observed above experiments, 1-MCP-treated fruits had higher fruit weight and firmness than those of control fruits, regardless of the delayed applications of 1-MCP.