Bacillus genus are found abundantly in various sites and their secondary metabolites were used as potential agents in agriculture, notably plant growth promoting and bio-control. The objective of this study was to develop the culture conditions of GH1-13 strain including higher cell growth, stable endospore-forming and enhancement of potential agents which are related with plant growth promoting and phytopathogen suppression. The optimal carbon and nitrogen sources were determined by glucose and soy bean flour, respectively, then resulted in 7.5×109 cells/mL, 6.8×109 endospore cells/mL and sporulation yield of 90% after 30 h cultivation in 500 L submerged fermenter at 37℃, pH 7.0. Cells and cell-free supernatant of GH1-13 strains showed the potent antifungal activity against phytopathogenic fungi of Colletotrichum gloeosporioides. It was also confirmed that indole-3-acetic acid (IAA) production of GH1-13 strain was greatly increased by addition of 0.3% tryptophan.

Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 균주 및 배양 조건
  2. 최적 탄소원 및 질소원 선발
  3. 배지 최적화 및 균주 배양
  4. 최적배지를 이용한 대량배양
  5. 식물 병원성 곰팡이에 대한 항진균활성 검정
  6. Indole-3-acetic acid (IAA) 생산성
 Ⅲ. 결과 및 고찰
  1. 대량배양용 배지조성 최적화
  2. 최적배지를 이용한 대량배양 조건 확립
  3. 식물 병원성 곰팡이에 대한 항진균활성 검정
  4. IAA 최대 생산을 위한 배양기술 확립
 Ⅳ. 적 요
 References

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