During the last couple of decades, molecular techniques are widespread tools for the species diversity. However, their application to taxonomy provoked intense debates between traditional and molecular taxonomists. To prevent every kind of disagreement, it should be required to a threshold for standarizing the species delimitation. Here, we tested three species delimitation methods (ABGD, PTP and bPTP) and compared their results with morphospecies on the widest DNA barcoding dataset. Moreover, a possible threshold for species determination within the superfamily has been suggested. It is believed that it could be an important criterion in detection for hidden or cryptic species, tracing of variation, and can help in identification on immature stages of pests.

• Sora Kim(Lab. of insect biosystematics, Department of Agricultural biotechnology, Research Institute of Agriculture and life Sciences, Seoul National University)
• Seunghwan Lee(Lab. of insect biosystematics, Department of Agricultural biotechnology, Research Institute of Agriculture and life Sciences, Seoul National University)