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Effect of hemin and zinc on experimental colon carcinogenesis in mice KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Excessive intake of red meat has been associated with colon carcinogenesis. The effect of hemin and zinc on colon carcinogenesis was investigated in male ICR mice. After acclimation for 1 week, five-week-old mice received three s.c. injections (0-2nd weeks of the experiment) of azoxymethane (AOM; 10 mg/kg b.w.) weekly and were treated by 2% dextran sodium sulfate (DSS) in drinking water for the next 1 week to induce aberrant crypt foci (ACF). Mice were fed on AIN-76A purified rodent diet for 6 weeks. There were three experimental groups; control, hemin, and hemin + zinc groups. The daily oral doses of hemin and zinc were 534 mg/kg and 55 mg/kg b.w., respectively. After staining colonic mucosa with 0.2% methylene blue, the ACF, aberrant crypts (AC), and polyps were counted. Lipid peroxidation in liver was evaluated by thiobarbituric acid-reactive substances (TBARS) assay. The numbers of AC, ACF, large ACF (i.e., ≥4 AC/ACF), and polyps in the hemin group were higher than those in the control group. In hemin + zinc group, the numbers of AC, ACF, large ACF were significantly lower compared to the hemin or control groups (p < 0.05), and the number of polyps was also significantly lower compared to that in the hemin group (p < 0.05). The TBARS level in the livers of the hemin + zinc group was significantly lower than that of the hemin group (p < 0.05). These results suggest that hemin enhances colon carcinogenesis and that co-treatment with zinc can protect against the induction of colon carcinogenesis.

목차
Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Experimental materials
  Animals and Experiment diets
  Experiment design
  Sample collection
  ACF, AC and polyp count
  Thiobarbituric acid-reactive substances (TBARS) assay
  Statistical analysis
 RESULTS
  Changes in body weight
  Total aberrant crypt foci number
  Total aberrant crypt number
  Total number of large aberrant crypt foci
  Polyp numbers
  TBARS levels in liver
 DISCUSSION
 REFERENCES
저자
  • Hyo-Suk Choi(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Jun-Ho Kim(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Sang Yoon Nam(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Young Won Yun(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Jae-Hwang Jeong(Department of Biotechnology and Biomedicine, Osong Biocampus of Chungbuk Provincial University) Corresponding Authors
  • Beom Jun Lee(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) Corresponding Authors