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Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/371029
pp.1-5
In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and growth conditions
Expression and purification of recombinant protein
SDS-PAGE and Western Blot Analysis
Mice vaccination and B. abortus 544 challenge
Cytokine Quantification
Protective Experiments
Statistical analysis
RESULTS
Purification and immunogenicity of multiple recombinantproteins
Vaccination of multiple recombinant proteins protectsmice against Brucella infection
Determination of immune responses
DISCUSSION
REFERENCES
