The purpose of this study was to investigate the antioxidant activity and tyrosinase inhibitory activity of Codonopsis lanceolata 50% ethanol extract, and its solvent fractions (n-hexane, ethyl acetate (EA), n-butanol, water). The main components of the EA fraction were qualitatively analyzed using UPLC Q-ToF/MS. Additionally, a quantitative analysis was performed using UPLC. As a result, the total polyphenol content was 113.36 mg gallic acid/g in the EA fraction, which contained the largest amount of the C. lancolata solvent fractions. Also EA showed the highest antioxidant activity than other fractions. The IC50 of DPPH(2,2-diphenyl-1- picrylhydrazyl) radical scavenging activity was 0.03 mg/mL and the IC50 of ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity was 0.049 mg/mL. The EA fraction showed tyrosinase inhibitory activity than other fractions and especially inhibited monophenolase oxidase reaction higher than diphenolase oxidase reaction. The monophenolase oxidase inhibited 55% when the concentration of the EA fraction was 0.25 mg/mL. As a result of Q-ToF/MS analysis, it was confirmed that tangshenoside I and lobetyolin were the main components of EA fraction. Thus, these results suggest that C. lanceolata may be used as a potent source of cosmetic agents.

Abstract
서 론
재료 및 방법
    1. 재료 및 시약
    2. 추출물 및 용매분획물 제조
    3. 총 폴리페놀 및 총 플라보노이드 함량
    4. DPPH 및 ABTS 라디칼 소거 활성
    5. Mushroom tyrosinase 저해 활성
    6. 더덕 EA 분획물 정성 분석
    7. Tangshenoside I과 lobetyolin 정량 분석
    8. 통계처리
결과 및 고찰
    1. 총 폴리페놀 및 총 플라보노이드 함량 분석
    2. DPPH 및 ABTS 라디칼 소거 활성
    3. Mushroom tyrosinase 저해 활성
    4. 더덕 EA 분획물 정성 분석
    5. Tangshenoside I과 lobetyolin 정량 분석 및 tyrosinase저해활성
요약 및 결론
REFERENCES

• 김지영(농촌진흥청 국립농업과학원 농식품자원부) | Ji Yeong Kim (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
• 이민기(농촌진흥청 국립농업과학원 농식품자원부) | Min-Ki Lee (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
• 황병순(농촌진흥청 국립농업과학원 농식품자원부) | Byung Soon Hwang (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
• 김기창(농촌진흥청 국립농업과학원 농식품자원부 농업연구사) | Gi-Chang Kim (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
• 황인국(농촌진흥청 국립농업과학원 농식품자원부 농업연구사) | In Guk Hwang (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) Corresponding author