The purpose of this study was to develop synbiotic materials and to apply them to the puffed grain products using electrostatic spraying technology. Lactic acid bacteria were isolated from Kimchi and selected through tests of acid resistance, bile salt resistance and γ-aminobutyric acid (GABA) content. The isolated Lactobacillus brevis CFM21 produced highest GABA production up to a concentration of 926.42 μg/mL when grown in MRS broth containing 0.8% MSG. The possibility of coated grains as a prebiotic material was evaluated Confocal laser scanning micro scopy (CLSM). Rice bran extract containing 2% dextrose, 2% soytone, 0.2% potassium chloride, 0.6% MSG was produced 524.77 μg/mL of GABA. Citrus sinensis oil showed the highest antibacterial activity against Clostridium perfringens Electrostatic spray showed much higher effectiveness than conventional spray in coating the puffed grain product through CLSM. Applying Rice bran culture and Citrus sinensis oil to puffed grain product using electrostatic spray could contribute to promote intestinal health of consumers.

Abstract
Introduction
Objective
Materials and Methods
Results
Conclusion
References

• Min Su Jeong(Department of Food Science and Biotechnology, Chungbuk National University)
• Jeong Hyeon Oh(Department of Food Science and Biotechnology, Chungbuk National University)
• Chung Ha Lim(Department of Food Science and Biotechnology, Chungbuk National University)
• Jun Young Jeong(GD&Y Co., Ltd.)
• Hui Hyeon Park(GD&Y Co., Ltd.)
• Kwang Yup Kim(Department of Food Science and Biotechnology, Chungbuk National University)