Probiotic-derived bacteriocins have attracted considerable attention as an alternative to synthetic preservatives in the food industry, as synthetics are harmful to health. Lactobacillus gasseri G2 isolated from infant feces has been demonstrated to possess probiotic properties such as bile salt and acid tolerance, antibiotic resistance, and broad antimicrobial activity. Bacteriocin was partially purified by acetone precipitation and characterized for several properties. It was found to be highly thermostable and resistant to heating at 120oC for 15 min. Bacteriocin activity remained unaffected with changes in the pH range from 2.0 to 10.0 and by solvents such as acetone, methanol, ethanol, and butanol. The antimicrobial activity was not affected by α-amylase, lysozyme, and lipase, but was reduced after treatment with protease, trypsin, and proteinase K, suggesting that it has a proteinaceous nature. Bacteriocin inhibited a broad range of target bacteria belonging to both Gram-positive and Gram-negative groups: Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, Escherichia coli O157:H7, Enterobacter sakasakii, Salmonella Enteritidis, Shigella flexneri, Shigella boydii, Vibrio parahaemolyticus, and Vibrio vulnificus. Bacteriocin appeared as a smear of the protein band with a molecular weight between 2-5 kDa, which showed a clear zone of inhibition after bioassay. The high thermostability and acid/alkali stability of bacteriocin reflect its potential commercial significance.

Abstract
서 론
재료 및 방법
    박테리오신 생산 유산균의 분리 및 배양
    박테리오신 검출 및 확인
    박테리오신 생산 균주 동정
    균주 생장곡선과 박테리오신 생산
    박테리오신 생산균주의 프로바이오틱스 특성
    조항균성 물질의 제조
    박테리오신의 안정성 조사
    박테리오신 항균범위
    박테리오신 분자량 측정
결과 및 고찰
    균주의 선발과 동정
    프로바이오틱스 특성
    균주 생장곡선과 박테리오신 생산
    박테리오신의 안정성
    박테리오신의 항균범위
    박테리오신의 분자량
요 약
References

• 김종희(국립축산과학원 축산물이용과) | Jong-Hui Kim (National Institute of Animal Science, Rural Development Administration)
• 이은선(국립축산과학원 축산물이용과) | Eun-Seon Lee (National Institute of Animal Science, Rural Development Administration)
• 김부민(국립축산과학원 축산물이용과) | Bu-Min Kim (National Institute of Animal Science, Rural Development Administration)
• 함준상(국립축산과학원 축산물이용과) | Jun-Sang Ham (National Institute of Animal Science, Rural Development Administration)
• 오미화(국립축산과학원 축산물이용과) | Mi-Hwa Oh (National Institute of Animal Science, Rural Development Administration) Corresponding author