1. 본 연구는 GM콩이 비의도적으로 자연에 방출되었을 시 야생콩과의 교잡에 의해 발생 가능한 교잡콩을 모니터링하고 영양학적 측면에서 안전성을 평가하기 위하여 수행하였다. 이를 위해, 베타카로틴 강화 GM콩과 야생콩, 두 품종간 인위적으로 생산한 교잡콩, GM콩의 모본이자 일반 재배 품종인 광안콩 등 4품종을 국내 2개 지역에서 재배하고 콩 종자를 대상으로 주요 영양성분 38종을 분석하였다. 2. 재배 환경에 영향을 받지 않고, GM콩 및 야생콩과 비교해 교잡콩에서 유의적 차이를 보이는 성분은 조단백질, 조섬유, alanine, glycine, leucine, serine, tryptophan, oleic acid, linolenic acid, arachidonic acid 등 10종이었다. 하지만, 분석한 모든 시료의 성분은 국내외 자연 범위에 포함되는 수준이었다. 3. PCA분석 결과, 주요 영양성분 함량 변이는 GM콩은 모본과 유사하였고, 교잡콩은 GM콩 보다는 야생콩과 유사하였다. 즉, 분석한 4품종의 영양성분의 변이는 재배 환경보다는 유전적 요인에 의해 더 크게 영향을 받음을 알았다. 4. 본 연구의 결과는 GM콩과 야생콩간 교잡콩의 영양학적 안전성을 분석한 최초의 보고이며, 이는 향후 GM콩의 비의도적 자연 방출을 모니터링하는데 있어 유용한 기초 자료로써 활용 가치가 있을 것이다.

This study was conducted to evaluate the nutritional safety and monitor hybrid soybean that can occur due to hybridization between wild soybean and genetically modified (GM) soybean when GM soybean is unintentionally released to environment. For this, β-carotene-enhanced GM soybean, wild soybean, artificially produced hybrid soybean between the two varieties, and ‘Gwangan’, the parent cultivar of GM, are cultivated in two areas in Korea and 38 nutrients were analyzed from the seeds of four varieties. The nutrients that showed significant differences in hybrid soybeans compared to GM and wild soybeans which were not affected by the cultivation environment were crude protein, crude fiber, alanine, glycine, leucine, serine, tryptophan, oleic acid, linolenic acid, and arachidonic acid. However, the levels of all nutrients obtained from samples analyzed were within the natural variation. As a result of PCA analysis, it was found that the variation of nutrient composition of the four varieties analyzed was more affected by genetic factors than by environmental factors such as growing conditions. Taken together, the nutritional safety of hybrid soybean is not different from cultivated soybean and wild soybean, and ten nutrients showing significant differences are possible as important components for identifying and monitoring hybridized soybeans. To our knowledge, this is the first study to assess the nutritional safety of hybrid soybean developed by hybridization between GM and wild soybeans, and the results of this study will be valuable as basic data for monitoring GM soybean when the soybean is unintentionally released to environment.

ABSTRACT
 서 언
 재료 및 방법
  1. 실험 재료
  2. 일반 성분 분석
  3. 아미노산 분석
  4. 무기질 분석
  5. 지방산 분석
  6. 통계 분석
 결과 및 고찰
  1. 일반 성분 함량 분석
  2. 아미노산 함량 분석
  3. 무기질 함량 분석
  4. 지방산 조성 분석
  5. 주요 영양성분 함량의 PCA 분석
 적 요
 REFERENCES

• 오성덕(농촌진흥청 국립농업과학원 농업생명자원부) | Sung-Dug Oh (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
• 장예진(농촌진흥청 국립농업과학원 농업생명자원부) | Ye-Jin Jang (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
• 이경민(농촌진흥청 국립농업과학원 농업생명자원부) | Gyeong Min Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
• 이기종(농촌진흥청 국립농업과학원 농업생명자원부) | Kijong Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
• 서상재(경북대학교 농업생명과학대학 응용생명과학부) | Sang Jae Suh (School of Applied Biosciences, Kyungpook National University)
• 류태훈(농촌진흥청 국립농업과학원 농업생명자원부) | Tae-Hoon Ryu (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
• 박수윤(농촌진흥청 국립농업과학원 농업생명자원부) | Soo-Yun Park (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) Corresponding author